Department of Biophysics, Huygens laboratory of the State University, P.O. Box 9504, 2300 RA, Leiden.
Photosynth Res. 1991 Jun;28(3):149-53. doi: 10.1007/BF00054128.
Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-β-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.
用正十二烷基-β-D-麦芽糖苷孵育菠菜 PSII 膜片段，再通过快速一步阴离子交换分离，以 75%的得率从 PS II 核心颗粒中分离出纯的、有活性的、每反应中心含 35 个叶绿素的放氧 PS II 核心颗粒。用 Triton X-100 在柱子上处理这些颗粒，可将其以 55%的得率转化为纯的、有活性的 PS II 反应中心颗粒，其中每反应中心含有 6 个叶绿素。
