快速简便地从菠菜中分离纯光系统 II 核心和反应中心颗粒。
Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach.
机构信息
Department of Biophysics, Huygens laboratory of the State University, P.O. Box 9504, 2300 RA, Leiden.
出版信息
Photosynth Res. 1991 Jun;28(3):149-53. doi: 10.1007/BF00054128.
Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-β-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.
用正十二烷基-β-D-麦芽糖苷孵育菠菜 PSII 膜片段，再通过快速一步阴离子交换分离，以 75%的得率从 PS II 核心颗粒中分离出纯的、有活性的、每反应中心含 35 个叶绿素的放氧 PS II 核心颗粒。用 Triton X-100 在柱子上处理这些颗粒，可将其以 55%的得率转化为纯的、有活性的 PS II 反应中心颗粒，其中每反应中心含有 6 个叶绿素。
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