Hans Berger Department of Neurology, Friedrich-Schiller University, Jena, Germany.
Invest Ophthalmol Vis Sci. 2010 May;51(5):2686-96. doi: 10.1167/iovs.09-4537. Epub 2009 Dec 10.
The purpose of this in vivo study was to evaluate the proliferative response of immunologic cells during the acute phase after optic nerve (ON) lesion in the neural retina and the ciliary body (CB) in the adult mouse.
The number of cells obtained 5 to 10 days after ON crush was compared with that counted after intraorbital ON transection. In addition, after ON crush, the time course of in situ proliferating Ki67(+) microglia and macrophages was analyzed from 6 hours up to 10 days.
The number of BrdU(+)F4/80(+) retinal microglia and ciliary macrophages increased over time, reaching the peak number 10 days after ON lesion. In the retina, both ON lesion types resulted in a similar number of BrdU(+)F4/80(+) microglia. Approximately 85% of all BrdU(+) cells were identified as F4/80(+) microglia. However, this cell population represented only 30% of all F4/80(+) microglia. The peak of microglial in situ proliferation was found 2 days after ON crush. In the CB, both ON lesion types induced a significant increase in the number of BrdU(+)F4/80(+) macrophages. Of interest, the number of cells after ON transection further increased over time, whereas those after ON crush did not.
ON lesion significantly increased proliferation of F4/80(+) immunologic cells in both the retina and CB. Although no significant differences in cellular response were observed in the retina between both lesion types, ON transection had a more pronounced effect on ciliary macrophages than did ON crush. Therefore, both regions seem not to act in concert during the acute phase after ON lesion.
本体内研究旨在评估成年小鼠视神经（ON）损伤后神经视网膜和睫状体（CB）中免疫细胞的增殖反应。
将 ON 挤压后 5 至 10 天获得的细胞数量与视神经眶内横断后计数的细胞数量进行比较。此外，在 ON 挤压后，分析了 Ki67（+）小胶质细胞和巨噬细胞原位增殖的时间过程，时间范围为 6 小时至 10 天。
BrdU（+）F4/80（+）视网膜小胶质细胞和睫状巨噬细胞的数量随时间增加，在 ON 损伤后 10 天达到峰值。在视网膜中，两种 ON 损伤类型均导致 BrdU（+）F4/80（+）小胶质细胞数量相似。大约 85%的 BrdU（+）细胞被鉴定为 F4/80（+）小胶质细胞。然而，该细胞群体仅代表所有 F4/80（+）小胶质细胞的 30%。ON 挤压后 2 天发现小胶质细胞原位增殖的峰值。在 CB 中，两种 ON 损伤类型均导致 BrdU（+）F4/80（+）巨噬细胞数量显著增加。有趣的是，ON 横断后细胞数量随时间进一步增加，而 ON 挤压后则没有。
ON 损伤显著增加了视网膜和 CB 中 F4/80（+）免疫细胞的增殖。尽管两种损伤类型在视网膜中观察到的细胞反应没有明显差异，但 ON 横断对视神经睫状体巨噬细胞的影响比 ON 挤压更为明显。因此，在 ON 损伤后急性期，两个区域似乎没有协同作用。
