Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, USDA, Beltsville, MD 20705, USA.
Poult Sci. 2010 Jan;89(1):20-7. doi: 10.3382/ps.2009-00351.
Associations between the parameters of resistance to coccidiosis and SNP in 3 candidate genes located on chromosome 1 [T cell receptor-beta (TCR-beta), myeloid leukemia factor 2 (MLF2), and lymphotactin] were determined. Single nucleotide polymorphisms were genotyped in 24 F1 generation and 290 F2 generation birds. Four SNP were identified in the lymphotactin gene, 12 were located in the TCR-beta gene, and 4 in the MLF2 gene. At various times after experimental infection of the F2 generation with Eimeria maxima, BW, fecal oocyst shedding, and biochemical parameters were measured as parameters of coccidiosis resistance. Single marker association test was applied to determine the associations between the 20 SNP and the parameters of coccidiosis resistance. The maximum additive genetic effect on disease resistance of an SNP in MLF2 was explained by BW (P = 0.0002). The SNP in MLF2 significantly associated with BW was also associated with fecal oocyst shedding (P = 0.001). Four SNP associated with oocyst shedding were found within the coding region of TCR-beta (P < 0.05). Although none of the lymphotactin SNP were associated with oocyst shedding, interferon-gamma level was associated with 2 SNP in lymphotactin. These results suggest that the SNP in the MLF2 gene can be one of the markers to select coccidiosis resistance in chickens.
测定了位于第 1 号染色体上的 3 个候选基因[T 细胞受体-β（TCR-β）、髓样白血病因子 2（MLF2）和淋球菌趋化因子]上抗球虫病的抗性参数与 SNP 之间的关联。在 24 只 F1 代和 290 只 F2 代鸡中对单核苷酸多态性进行了基因分型。在淋球菌趋化因子基因中鉴定出 4 个 SNP，TCR-β 基因中有 12 个，MLF2 基因中有 4 个。在 F2 代用柔嫩艾美耳球虫感染后的不同时间，测量 BW、粪便卵囊脱落和生化参数作为抗球虫病的抗性参数。应用单标记关联试验确定 20 个 SNP 与抗球虫病参数之间的关联。MLF2 中 SNP 对疾病抗性的最大加性遗传效应由 BW 解释（P=0.0002）。与 BW 显著相关的 MLF2 中的 SNP 也与粪便卵囊脱落相关（P=0.001）。在 TCR-β 的编码区发现与卵囊脱落相关的 4 个 SNP（P<0.05）。尽管与卵囊脱落相关的淋球菌趋化因子 SNP 没有，但干扰素-γ水平与淋球菌趋化因子中的 2 个 SNP 相关。这些结果表明，MLF2 基因中的 SNP 可以成为选择鸡抗球虫病的标记之一。
