Kim E-S, Hong Y H, Min W, Lillehoj H S
Department of Animal Sciences, University of Wisconsin-Madison, 53706, USA.
Poult Sci. 2006 Nov;85(11):2028-30. doi: 10.1093/ps/85.11.2028.
Two commercial, pure broiler lines with different susceptibility to coccidiosis were used to fine-map QTL associated with the previously identified marker LEI0101, located at 259 cM on chromosome 1 and shown to be significantly associated with disease resistance. Eight additional microsatellite markers linked to LEI0101 were used for genotyping of F(1) parents and F(2) offspring (n = 314), and their associations with oocyst shedding, as a marker of disease resistance, were determined in birds experimentally infected with Eimeria maxima. Single-point analysis of 4 families showed that logarithm of odds (LOD) scores at all marker loci were > 0.5, with the exception of marker LEI0071, located at 242 cM (LOD score = 2.45). Multipoint analysis showed a maximum LOD score between LEI0071 and LEI0101 at 254 cM (LOD score = 3.74). Although the LEI0071 marker was mapped near LEI0101 by linkage analysis, the physical location of LEI0071 was not identified. Further studies to determine the physical locations of these and other markers will allow additional application association mapping techniques using single nucleotide polymorphism markers.
选用两个对球虫病易感性不同的商用纯肉鸡品系，对与先前鉴定的位于1号染色体259厘摩处的标记LEI0101相关的数量性状基因座（QTL）进行精细定位，该标记已显示与抗病性显著相关。另外八个与LEI0101连锁的微卫星标记用于F(1)代亲本和F(2)代后代（n = 314）的基因分型，并在实验感染巨型艾美耳球虫的鸡中确定它们与作为抗病性标记的卵囊排泄的关联。对4个家系的单点分析表明，除了位于242厘摩处的标记LEI0071（LOD值 = 2.45）外，所有标记位点的优势对数（LOD）得分均> 0.5。多点分析显示，在254厘摩处LEI0071和LEI0101之间的最大LOD值为3.74。尽管通过连锁分析将LEI0071标记定位在LEI0101附近，但未确定LEI0071的物理位置。进一步确定这些标记和其他标记物理位置的研究将允许使用单核苷酸多态性标记进行更多应用关联图谱技术。
