Hong Yeong Ho, Kim Eui-Soo, Lillehoj Hyun S
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Beltsville, MD 20705, USA.
BMC Proc. 2011 Jun 3;5 Suppl 4(Suppl 4):S21. doi: 10.1186/1753-6561-5-S4-S21.
MLF2 was the candidate gene associated with coccidiosis resistance in chickens. Although single marker analysis supported the association between MLF2 and coccidiosis resistance, causative mutation relevant to coccidiosis was not identified yet. Thus, this study suggested segregation analysis of MLF2 haplotype and the association test of the other candidate genes using improved data transformation.
A haplotype probably originated from one parental line was found out of 4 major haplotypes of MLF2. Frequency of this haplotype was 0.2 in parental chickens and its offspring in 12 families. Allele substitution effect of the MLF2 haplotype originated from a specific line was associated with increased body weight and fecal egg count explaining coccidiosis resistance. Nevertheless Box-Cox transformation was able to improve normality; association test did not produce obvious different results compared with analysis with log transformed phenotype.
Allele substitution effect analysis and classification of MLF2 haplotype identified the segregation of haplotype associated with coccidiosis resistance. The haplotype originated from a specific parental line was associated with improving disease resistance. Estimating effect of MLF2 haplotype on coccidiosis resistance will provide useful information for selecting animals or lines for future study.
MLF2是与鸡抗球虫病相关的候选基因。尽管单标记分析支持MLF2与抗球虫病之间的关联，但尚未确定与球虫病相关的致病突变。因此，本研究建议使用改进的数据转换对MLF2单倍型进行分离分析，并对其他候选基因进行关联测试。
在MLF2的4种主要单倍型中发现了一种可能起源于一个亲本系的单倍型。该单倍型在12个家系的亲本鸡及其后代中的频率为0.2。源自特定品系的MLF2单倍型的等位基因替代效应与体重增加和粪便卵数增加相关，这解释了抗球虫病的现象。然而，Box-Cox转换能够改善正态性；与对数转换表型分析相比，关联测试并未产生明显不同的结果。
MLF2单倍型的等位基因替代效应分析和分类确定了与抗球虫病相关的单倍型的分离。源自特定亲本系的单倍型与提高抗病性相关。估计MLF2单倍型对球虫病抗性的影响将为未来研究选择动物或品系提供有用信息。
