School of Chemistry, Jilin University, Changchun, People's Republic of China.
Nanotechnology. 2010 Jan 29;21(4):045501. doi: 10.1088/0957-4484/21/4/045501. Epub 2009 Dec 10.
A novel fluorescent nanosensor for the determination of Cu(2+) was synthesized with N-(quinoline-8-yl)-2-(3-triethoxysilyl-propylamino)-acetamide (QlOEt) grafted onto the surface of silica nanoparticles (SiNPs) using the reverse microemulsion method. Spherical SiNPs were used as substrate and QlOEt was used simultaneously as the binding and readout system for Cu(2+). This sensor has been realized as a highly sensitive and selective technique for the detection and quantification of trace amounts of Cu(2+). The probe exhibits a dynamic response range for Cu(2+) from 2.0 x 10(-6) to 2.0 x 10(-5) M, with a detection limit of 3.8 x 10(-7) M. Other alkali, alkaline earth, and transitional metal ions including Li(+), K(+), Mg(2+), Ca(2+), Sr(2+), Mn(2+), Zn(2+), Mo(6+), Pb(2+), Ag(+) had no significant interference on Cu(2+) determination. Poisonous and flammable reagents are avoided during the synthesis of this nanosensor. Therefore the strategy explored in this work can be extended to the synthesis of other chemo- and biosensors for direct detection of specific targets in an intracellular environment.
一种新型荧光纳米传感器用于测定 Cu(2+)，是通过反胶束法将 N-(8-喹啉基)-2-(3-三乙氧基硅丙基氨基)乙酰胺 (QlOEt) 接枝到硅纳米粒子 (SiNPs) 表面而合成的。球形 SiNPs 用作基质，同时 QlOEt 用作 Cu(2+)的结合和读出系统。该传感器已成为一种用于检测和定量痕量 Cu(2+)的高灵敏度和选择性技术。该探针对 Cu(2+)的动态响应范围为 2.0 x 10(-6)至 2.0 x 10(-5) M，检测限为 3.8 x 10(-7) M。其他碱金属、碱土金属和过渡金属离子，包括 Li(+)、K(+)、Mg(2+)、Ca(2+)、Sr(2+)、Mn(2+)、Zn(2+)、Mo(6+)、Pb(2+)、Ag(+)，对 Cu(2+)的测定没有明显干扰。在合成这种纳米传感器时避免了有毒和易燃试剂的使用。因此，本工作中探索的策略可以扩展到其他化学和生物传感器的合成，用于直接在细胞内环境中检测特定目标。
