College of Food Science and Engineering, Jilin University, No. 5333 Xi'an Road, Changchun, 130062, China.
Sci Rep. 2022 Apr 22;12(1):6624. doi: 10.1038/s41598-022-10500-9.
Biomolecule-functionalized fluorescent gold nanocluster (AuNCs) have attracted a lot of attention due to good biocompatibility, stable physicochemical properties and considerable cost advantages. Inappropriate concentration of Cu may cause a variety of diseases. In this study, AuNCs were synthesized in alkaline aqueous solution using bovine serum albumin (BSA) as a template. And then, the peptide CCYWDAHRDY was coupled to AuNCs. Furthermore, the fluorescence of synthesized CCYWDAHRDY-AuNCs response to Cu was evaluated. As the results shown that the CCYWDAHRDY-AuNCs can sensitively detect Cu. After adding Cu to the probe system, the fluorescence of the CCYWDAHRDY-AuNCs was quenched. The detection conditions were at pH 6 and 30 °C for 10 min, the linear relationship between Cu concentration and fluorescence intensity were good in the range of 0.1 ~ 4.2 μmol/L. The regression equation was y = - 105.9x + 693.68, the linear correlation coefficient is 0.997, and the minimum detection limit was 52 nmol/L.
生物分子功能化荧光金纳米簇 (AuNCs) 由于良好的生物相容性、稳定的物理化学性质和相当大的成本优势而受到广泛关注。Cu 的浓度不当可能会导致各种疾病。在这项研究中，使用牛血清白蛋白 (BSA) 作为模板，在碱性水溶液中合成了 AuNCs。然后，将肽 CCYWDAHRDY 偶联到 AuNCs 上。此外，还评估了合成的 CCYWDAHRDY-AuNCs 对 Cu 的荧光响应。结果表明，CCYWDAHRDY-AuNCs 可以灵敏地检测 Cu。向探针体系中加入 Cu 后，CCYWDAHRDY-AuNCs 的荧光被猝灭。检测条件为 pH 6 和 30°C 10 min，在 0.1~4.2 μmol/L 范围内 Cu 浓度与荧光强度之间具有良好的线性关系。回归方程为 y=-105.9x+693.68，线性相关系数为 0.997，检测限最低为 52 nmol/L。
