Imura Yuki, Asano Yasuyuki, Sato Kiichi, Yoshimura Etsuro
Department of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
Anal Sci. 2009 Dec;25(12):1403-7. doi: 10.2116/analsci.25.1403.
Intestinal absorption rates vary with the nature of the substances involved. In-vitro experiments with cell culture inserts are often conducted to evaluate the intestinal absorption rate. These inserts, however, require large amounts of cells, samples, and culture media, and take a long time to evaluate. To overcome these problems, we developed a microchip-based system that mimics the intestine. The microchip was composed of a glass slide, a permeable membrane, and polydimethylsiloxane (PDMS) sheets, which contained microchannels made by photolithography; Caco-2 cells were cultured on the membrane in the microchip. The system was regulated with a microsyringe pump. We conducted permeation tests; cyclophosphamide, which can permeate the intestinal barrier, displayed a high permeability coefficient and Lucifer yellow, which cannot be absorbed at the intestinal wall, displayed a low permeability coefficient. These results were consistent with those obtained using a conventional method, which supports the validity of our new system. The system realized an 80% reduction of cell consumption.
肠道吸收速率因所涉及物质的性质而异。常通过使用细胞培养插入物进行体外实验来评估肠道吸收速率。然而，这些插入物需要大量的细胞、样本和培养基，并且评估耗时较长。为克服这些问题，我们开发了一种基于微芯片的模拟肠道系统。该微芯片由载玻片、渗透膜和聚二甲基硅氧烷（PDMS）片组成，其中包含通过光刻法制成的微通道；Caco-2细胞培养在微芯片内的膜上。该系统由微量注射泵调节。我们进行了渗透测试；可透过肠道屏障的环磷酰胺显示出高渗透系数，而不能在肠壁吸收的荧光素黄显示出低渗透系数。这些结果与使用传统方法获得的结果一致，这支持了我们新系统的有效性。该系统实现了细胞消耗减少80%。
