Feinberg R F, Kliman H J, Lockwood C J
Department of Obstetrics and Gynecology, University of Pennsylvania Medical Center, Philadelphia 19104-4283.
Am J Pathol. 1991 Mar;138(3):537-43.
Using an antibody probe specific for the class of fibronectins that contain the oncofetal domain, it was shown that oncofetal fibronectin (onfFN) is present wherever trophoblasts make contact with extraplacental extracellular matrix (ECM). In normal human implantation sites, onfFN was localized to a highly specific region-the ECM connecting extravilous trophoblasts and trophoblastic cell columns to the uterine decidua. This same zone of onfFN was present in an analogous location in extrauterine gestations. Like these in vivo extravillous trophoblasts, isolated cytotrophoblasts in primary culture synthesized and secreted onfFN as they underwent differentiation. Furthermore, when cocultured with an ECM gel, cytotrophoblast aggregates deposited onfFN at cell-ECM contact sites, resembling early implanting trophoblasts in vivo. In the presence of cyclic AMP agonists, onfFN synthesis was inhibited markedly. It is concluded from these results that onfFN is a trophoblast protein that, under cAMP regulation, could mediate implantation and placental-uterine attachment throughout gestation.
使用针对含有癌胚结构域的纤连蛋白类别的抗体探针，研究表明，只要滋养层细胞与胎盘外细胞外基质（ECM）接触，癌胚纤连蛋白（onfFN）就会存在。在正常人类着床部位，onfFN定位于一个高度特定的区域——将绒毛外滋养层细胞和滋养层细胞柱与子宫蜕膜连接起来的ECM。在宫外妊娠的类似位置也存在相同的onfFN区域。与这些体内绒毛外滋养层细胞一样，原代培养中分离的细胞滋养层细胞在分化过程中合成并分泌onfFN。此外，当与ECM凝胶共培养时，细胞滋养层聚集体在细胞与ECM接触部位沉积onfFN，类似于体内早期着床的滋养层细胞。在环磷酸腺苷（cAMP）激动剂存在的情况下，onfFN的合成受到显著抑制。从这些结果可以得出结论，onfFN是一种滋养层蛋白，在cAMP调节下，它可能在整个妊娠期介导着床和胎盘与子宫的附着。
