Department of Morphology, Stomatology and Physiology, Dentistry School, University of São Paulo-USP, 14040-904, Ribeirão Preto, São Paulo, Brazil.
Implant Dent. 2009 Dec;18(6):521-9. doi: 10.1097/ID.0b013e3181b8e53c.
Because of limitations of autogenous grafts, allografts, xenografts, alloplasts, and hydroxyapatite as graft materials, researchers have been using bone tissue engineering as a strategy for bone regeneration. The aim of this work was to study the effect of bone tissue engineering, associating bone marrow osteoblastic cells, and autogenous bone in defects created by dental extraction in rats.
Eighty male rats from 250 to 300 g were anesthetized, submitted to the extraction of the superior incisor, and divided in control group (C), implanted with osteoblastic cells (OC), autogenous bone (AB), and osteoblastic cells + autogenous bone (OC + AB). The animals were killed on 10th and 20th days after surgery and their maxilla were processed for obtaining fine semiserial sections (5 mum), and then stained with hematoxylin-eosin. Through image analysis system, bone volume in areas adjacent to the implants was estimated.
The histometric results revealed that the association OC + AB produced significant increase (10%-15%) of bone in both experimental periods when compared with the control group (P < or = 0.01).
Osteoblastic cells associated with autogenous bone accelerated the repair of bone defect, and the action of the osteoblastic cells was more effective until the 10th day and of the autogenous bone after this period.
由于自体移植物、同种异体移植物、异种移植物、异体移植物和羟磷灰石等移植物材料的局限性，研究人员一直将骨组织工程作为骨再生的一种策略。本研究旨在探讨骨组织工程结合骨髓成骨细胞和自体骨在大鼠拔牙创中对骨再生的影响。
80 只体重为 250 至 300 克的雄性大鼠接受麻醉，上颌切牙被拔除，并分为对照组（C）、成骨细胞植入组（OC）、自体骨植入组（AB）和成骨细胞+自体骨植入组（OC+AB）。术后第 10 天和第 20 天处死动物，对其上颌进行处理，获得精细的半连续切片（5 微米），然后用苏木精-伊红染色。通过图像分析系统，估计植入物周围区域的骨体积。
组织学结果显示，与对照组相比（P≤0.01），在两个实验期，OC+AB 联合均显著增加（10%-15%）骨量。
成骨细胞与自体骨联合应用加速了骨缺损的修复，成骨细胞的作用在第 10 天之前更为有效，而自体骨的作用则在此之后。
