人乳头瘤病毒 16 型和 18 型的 mRNA 水平而非 DNA 水平可能与宫颈癌的进展阶段有关。
Human papillomavirus types 16 and 18 mRNA levels and not DNA levels may be associated with advancing stages of cervical cancer.
机构信息
Department of Clinical Virology, Christian Medical College, Vellore, India.
出版信息
Int J Gynecol Cancer. 2009 Nov;19(8):1415-20. doi: 10.1111/IGC.0b013e3181b62e05.
OBJECTIVE
Human papillomavirus (HPV) contributes to the development of cervical cancer. We hypothesize that HPV DNA and messenger RNA (mRNA) levels may be associated with increasing stages of cervical cancer.
MATERIALS AND METHODS
In this study, we measured DNA and mRNA viral loads of the most common high-risk HPV-16 and HPV-18 in cervical biopsy tissue of women with cervical neoplasia using real-time polymerase chain reaction.
RESULTS
Median HPV-16 and HPV-18 DNA viral loads were 58,342 copies and 71,367 per 5000 cells, respectively. We found that HPV-16 and HPV-18 DNA levels did not correlate with advancing tumor stage (P = 0.977 and P = 0.263). Messenger RNA transcripts were detected in 81 (86%) of HPV-16 DNA-positive women and in 16 (84.2%) of HPV-18-positive women. Median HPV-16 and HPV-18 transcript copy numbers were 5964 and 6158, respectively. In women with squamous cell carcinoma, HPV-16 mRNA loads showed an increasing but not statistically significant trend with advancing disease stage (rho = 0.231, P = 0.058).
CONCLUSIONS
We conclude that HPV mRNA levels and not DNA levels may be associated with advancing stages of cervical cancer.
目的
人乳头瘤病毒（HPV）是宫颈癌的致病因素。我们假设 HPV 的 DNA 和信使 RNA（mRNA）水平可能与宫颈癌的进展阶段有关。
材料与方法
在这项研究中，我们使用实时聚合酶链反应，对患有宫颈癌前病变的女性宫颈活检组织中最常见的高危型 HPV-16 和 HPV-18 的 DNA 和 mRNA 病毒载量进行了测量。
结果
HPV-16 和 HPV-18 的 DNA 病毒载量中位数分别为每 5000 个细胞 58342 拷贝和 71367 拷贝。我们发现 HPV-16 和 HPV-18 的 DNA 水平与肿瘤进展阶段没有相关性（P = 0.977 和 P = 0.263）。在 81 名 HPV-16 DNA 阳性的女性和 16 名 HPV-18 阳性的女性中检测到了信使 RNA 转录本。HPV-16 和 HPV-18 的转录本拷贝数中位数分别为 5964 和 6158。在患有鳞状细胞癌的女性中，HPV-16 mRNA 载量随着疾病阶段的进展呈上升趋势，但没有统计学意义（rho = 0.231，P = 0.058）。
结论
我们得出结论，HPV 的 mRNA 水平而非 DNA 水平可能与宫颈癌的进展阶段有关。
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