Syed Anees A, Lahiri Shibani, Mohan Divya, Valicherla Guru R, Gupta Anand P, Kumar Sudhir, Maurya Rakesh, Bora Himanshu K, Hanif Kashif, Gayen Jiaur R
Division of Pharmacokinetics and Metabolism, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
Division of Pharmacology, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
Front Pharmacol. 2016 Dec 21;7:510. doi: 10.3389/fphar.2016.00510. eCollection 2016.
Planchon (Family: Ulmaceae), a traditional medicinal plant, was used in fracture healing in the folk tradition of Uttarakhand, Himalaya, India. The present study investigated the cardioprotective effect of ethanolic extract (EE) and butanolic fraction (BF) of in isoprenaline (ISO) induced cardiac hypertrophy in Wistar rats. Cardiac hypertrophy was induced by ISO (5 mg/kg/day, subcutaneously) in rats. Treatment was performed by oral administration of EE and BF of (500 and 50 mg/kg/day). The blood pressure (BP) and heart rate (HR) were measured by non-invasive blood pressure measurement technique. Plasma renin, Ang II, NO, and cGMP level were estimated using an ELISA kit. Angiotensin converting enzyme activity was estimated. BP and HR were significantly increased in ISO group (130.33 ± 1.67 mmHg vs. 111.78 ± 1.62 mmHg, < 0.001 and 450.51 ± 4.90 beats/min vs. 347.82 ± 6.91 beats/min, respectively, < 0.001). The BP and HR were significantly reduced (EE: 117.53 ± 2.27 mmHg vs. 130.33 ± 1.67 mmHg, < 0.001, BF: 119.74 ± 3.32 mmHg vs. 130.33 ± 1.67 mmHg, < 0.001); HR: (EE: 390.22 ± 8.24 beats/min vs. 450.51 ± 4.90 beats/min, < 0.001, BF: 345.38 ± 6.79 beats/min vs. 450.51 ± 4.90 beats/min, < 0.001) after the treatment of EE and BF of , respectively. Plasma renin, Ang II, ACE activity was decreased and NO, cGMP level were increased. The EE and BF of down regulated the expression of ANP, BNP, TNF-α, IL-6, MMP9, β1-AR, TGFβ1 and up regulated NOS3, ACE2 and Mas expression level, respectively. Thus, this study demonstrated that has cardioprotective effect against ISO induced cardiac hypertrophy.
印度喜马拉雅地区北阿坎德邦的民间传统中，传统药用植物榆科朴属植物（Planchon，属榆科）被用于促进骨折愈合。本研究调查了榆科朴属植物的乙醇提取物（EE）和丁醇馏分（BF）对异丙肾上腺素（ISO）诱导的Wistar大鼠心脏肥大的心脏保护作用。通过给大鼠皮下注射ISO（5 mg/kg/天）诱导心脏肥大。通过口服榆科朴属植物的EE和BF（500和50 mg/kg/天）进行治疗。使用无创血压测量技术测量血压（BP）和心率（HR）。使用ELISA试剂盒估计血浆肾素、血管紧张素II、一氧化氮（NO）和环磷酸鸟苷（cGMP）水平。估计血管紧张素转换酶活性。ISO组的BP和HR显著升高（分别为130.33±1.67 mmHg对111.78±1.62 mmHg，P<0.001和450.51±4.90次/分钟对347.82±6.91次/分钟，P<0.001）。分别用榆科朴属植物的EE和BF治疗后，BP和HR显著降低（EE：117.53±2.27 mmHg对130.33±1.67 mmHg，P<0.001，BF：119.74±3.32 mmHg对130.33±1.67 mmHg，P<0.001）；HR：（EE：390.22±8.24次/分钟对450.51±4.90次/分钟，P<0.001，BF：345.38±6.79次/分钟对450.51±4.90次/分钟，P<0.001）。血浆肾素、血管紧张素II、ACE活性降低，NO、cGMP水平升高。榆科朴属植物的EE和BF分别下调心房钠尿肽（ANP）、脑钠肽（BNP）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、基质金属蛋白酶9（MMP9）、β1-肾上腺素能受体（β1-AR）、转化生长因子β1（TGFβ1） 的表达，并上调一氧化氮合酶3（NOS3）、血管紧张素转换酶2（ACE2）和Mas的表达水平。因此，本研究表明榆科朴属植物对ISO诱导的心脏肥大具有心脏保护作用。
