Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, Boston, Massachusetts, USA.
Nat Methods. 2010 Jan;7(1):53-5. doi: 10.1038/nmeth.1409. Epub 2009 Dec 13.
The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.
诱导多能干细胞(iPSCs)的产生通常涉及将重编程因子通过病毒导入体细胞。在这里,我们使用基因靶向技术生成了一种带有单个可诱导、多顺反子重编程盒的小鼠品系,从而可以在各种体细胞类型中诱导多能性。由于这些“可重编程的小鼠”很容易繁殖,因此它们是研究细胞重编程背后机制的有用工具。