Atyame Nten Celestine Michelle, Sommerer Nicolas, Rofidal Valerie, Hirtz Christophe, Rossignol Michel, Cuny Gerard, Peltier Jean-Benoit, Geiger Anne
UMR 177, IRD-CIRAD, CIRAD TA A-17 / G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.
J Biomed Biotechnol. 2010;2010:212817. doi: 10.1155/2010/212817.
Trypanosoma secretome was shown to be involved in parasite virulence and is suspected of interfering in parasite life-cycle steps such as establishment in the Glossina midgut, metacyclogenesis. Therefore, we attempted to identify the proteins secreted by procyclic strains of T. brucei gambiense and T. brucei brucei, responsible for human and animal trypanosomiasis, respectively. Using mass spectrometry, 427 and 483 nonredundant proteins were characterized in T. brucei brucei and T. brucei gambiense secretomes, respectively; 35% and 42% of the corresponding secretome proteins were specifically secreted by T. brucei brucei and T. brucei gambiense, respectively, while 279 proteins were common to both subspecies. The proteins were assigned to 12 functional classes. Special attention was paid to the most abundant proteases (14 families) because of their potential implication in the infection process and nutrient supply. The presence of proteins usually secreted via an exosome pathway suggests that this type of process is involved in trypanosome ESP secretion. The overall results provide leads for further research to develop novel tools for blocking trypanosome transmission.
锥虫分泌组被证明与寄生虫毒力有关，并且怀疑其干扰寄生虫生命周期的各个步骤，如在舌蝇中肠的定殖、循环后期发育。因此，我们试图鉴定分别导致人类和动物锥虫病的布氏冈比亚锥虫和布氏布氏锥虫前循环株分泌的蛋白质。通过质谱分析，在布氏布氏锥虫和布氏冈比亚锥虫分泌组中分别鉴定出427种和483种非冗余蛋白质；相应分泌组蛋白质中分别有35%和42%是布氏布氏锥虫和布氏冈比亚锥虫特异性分泌的，而有279种蛋白质是两个亚种共有的。这些蛋白质被归为12个功能类别。由于其在感染过程和营养供应中的潜在作用，我们特别关注了最丰富的蛋白酶（14个家族）。通常通过外泌体途径分泌的蛋白质的存在表明，这种类型的过程参与了锥虫的细胞外分泌产物（ESP）分泌。总体结果为进一步研究开发阻断锥虫传播的新工具提供了线索。
