Alvarez Adalucy, Moncayo José Ignacio, Santacruz Jorge Javier, Corredor Luisa Fernanda, Reinosa Elizabeth, Martínez José William, Beltrán Leonardo
Laboratorio de Microbiología y Parasitología, Centro de Biología Molecular y Biotecnología, Departamento de Ciencias Básicas, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira, Colombia.
Rev Med Chil. 2009 Oct;137(10):1309-14.
Helicobacter pylori antimicrobial resistance rates differ among countries and even between different areas of a country. In Colombia, the most commonly used antimicrobials for the treatment of H pylori infection are amoxicillin, clarithromycin and metronidazole.
To determine antimicrobial susceptibility of H pylori strains isolated in Colombia.
Eighty eight strains of H pylori were isolated and identified by microbiological methods and confirmed with polymerase chain reaction (PCR). The detection of antimicrobial resistance to amoxicillin, clarithromycin, metronidazole and tetraclycline, was conducted by the Etest method. Mutations in the 23S rDNA, involved in resistance to clarithromycin, were detected using PCR and restriction fragment length polymorphism.
Eighty eight and 2.2% of the strains were resistant to metronidazole and clarithromycin, respectively. No isolate was simultaneously resistant to amoxicillin or tetracycline. The two clarithromycin resistant strains were homozygous for the A2143G mutation. No mutations were found in the remaining 86 susceptible strains.
The high rate of metronidazole resistance in our population precludes the use of this drug for the empirical treatment of H pylori infection.
幽门螺杆菌的抗菌药物耐药率在不同国家甚至在一个国家的不同地区之间都存在差异。在哥伦比亚，治疗幽门螺杆菌感染最常用的抗菌药物是阿莫西林、克拉霉素和甲硝唑。
确定在哥伦比亚分离出的幽门螺杆菌菌株的抗菌药物敏感性。
通过微生物学方法分离并鉴定了88株幽门螺杆菌，并用聚合酶链反应（PCR）进行了确认。采用Etest法检测对阿莫西林、克拉霉素、甲硝唑和四环素的抗菌药物耐药性。使用PCR和限制性片段长度多态性检测与克拉霉素耐药相关的23S rDNA中的突变。
分别有88株和2.2%的菌株对甲硝唑和克拉霉素耐药。没有分离株同时对阿莫西林或四环素耐药。两株克拉霉素耐药菌株为A2143G突变纯合子。其余86株敏感菌株未发现突变。
我们研究人群中甲硝唑的高耐药率排除了将该药物用于幽门螺杆菌感染经验性治疗的可能性。
