Punctual mutations in gene of clarithromycin-resistant in Colombian populations.

AIM

To characterize punctual mutations in gene of clarithromycin-resistant () and determine their association with therapeutic failure.

METHODS

PCR products of gene V domain of 74 isolates; 34 resistant to clarithromycin (29 from a low-risk gastric cancer (GC) population: Tumaco-Colombia, and 5 from a high-risk population: Tuquerres-Colombia) and 40 from a susceptible population (28 from Tumaco and 12 from Túquerres) were sequenced using capillary electrophoresis. The concordance between mutations of V domain gene of and therapeutic failure was determined using the coefficient and McNemar's test was performed to determine the relationship between mutations and clarithromycin resistance.

RESULTS

gene from was amplified in 56/74 isolates, of which 25 were resistant to clarithromycin (20 from Tumaco and 5 from Túquerres, respectively). In 17 resistant isolates (13 from Tumaco and 4 from Túquerres) the following mutations were found: A1593T1, A1653G2, C1770T, C1954T1, and G1827C in isolates from Tumaco, and A2144G from Túquerres. The mutations T2183C, A2144G and C2196T in isolates resistant to clarithromycin from Colombia are reported for the first time. No association between the mutations and clarithromycin resistance was found. However, therapeutic failure of eradication treatment was associated with mutations of gene in clarithromycin-resistant ( = 0.71).

CONCLUSION

The therapeutic failure of eradication treatment in the two populations from Colombia was associated with mutations of the gene in clarithromycin-resistant .