Catholic University of the Sacred Heart, Largo A. Gemelli 8, Rome, Italy.
Pituitary. 2010 Jun;13(2):138-45. doi: 10.1007/s11102-009-0213-z.
The role of adrenal steroids in antioxidant regulation is not known. Previously, we demonstrated some Coenzyme Q(10) (CoQ(10)) alterations in pituitary diseases, which can induce complex pictures due to alterations of different endocrine axes. Therefore we determined CoQ(10) and Total Antioxidant Capacity (TAC) in pituitary-dependent adrenal diseases: 6 subjects with ACTH-dependent adrenal hyperplasia (AH); 19 with secondary isolated hypoadrenalism (IH), 19 with associated hypothyroidism (multiple pituitary deficiencies, MPH). CoQ(10) was assayed by HPLC; TAC by the system metmyoglobin-H(2)O(2), which, interacting with the chromogenous 2,2(I)-azinobis-(3-ethylbenzothiazoline-6-sulphonate), generates a spectroscopically revealed radical compound after a latency time (Lag) proportional to the antioxidant content. CoQ(10) levels were significantly lower in IH than AH and MPH, with a similar trend when adjusted for cholesterol. Also TAC was lower in IH than in AH and MPH, suggesting that adrenal hormones can influence antioxidants. However, since thyroid hormones modulate CoQ(10) levels and metabolism, when thyroid deficiency coexists it seems to play a prevalent influence.
肾上腺类固醇在抗氧化调节中的作用尚不清楚。之前，我们已经证明了一些在垂体疾病中的辅酶 Q10（CoQ10）改变，这些改变可能由于不同内分泌轴的改变而导致复杂的情况。因此，我们测定了垂体依赖性肾上腺疾病中的 CoQ10 和总抗氧化能力（TAC）：6 例 ACTH 依赖性肾上腺增生（AH）；19 例继发性孤立性肾上腺功能减退症（IH）；19 例伴有甲状腺功能减退症（多垂体缺乏症，MPH）。CoQ10 通过 HPLC 进行测定；TAC 通过 metmyoglobin-H2O2 系统进行测定，该系统与显色的 2,2(I)-氮杂双（3-乙基苯并噻唑啉-6-磺酸）相互作用，在与抗氧化剂含量成比例的潜伏期（Lag）后产生光谱上可检测到的自由基化合物。IH 中的 CoQ10 水平明显低于 AH 和 MPH，而调整胆固醇后则呈现出相似的趋势。IH 中的 TAC 也低于 AH 和 MPH，这表明肾上腺激素可以影响抗氧化剂。然而，由于甲状腺激素调节 CoQ10 水平和代谢，当甲状腺功能减退症并存时，它似乎发挥了主要影响。
