Yuan Chunhua, Jin Qihui, Tang Xing, Hu Weijun, Cao Rui, Yang Shengqing, Xiong Jixian, Xie Chunliang, Xie Jinyun, Liang Songping
Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, Life Science College, Hunan Normal University, Changsha 410081, People's Republic of China.
J Proteome Res. 2007 Jul;6(7):2792-801. doi: 10.1021/pr0700192. Epub 2007 Jun 14.
The bird spider Ornithoctonus huwena Wang is a very venomous spider in China. Several compounds with different types of biological activities have been identified previously from the venom of this spider. In this study, we have performed a proteomic and peptidomic analysis of the venom. The venom was preseparated into two parts: the venom proteins with molecular weight (MW) higher than 10,000 and the venom peptides with MW lower than 10 000. Using one-dimensional gel electrophoresis (1-DE), two-dimensional gel electrophoresis (2-DE), and mass spectrometry, 90 proteins were identified, including some important enzymes, binding proteins, and some proteins with significant biological functions. For venom peptides, a combination of cation-exchange and reversed-phase chromatography was employed. More than 100 components were detected by mass spectrometry, and 47 peptides were sequenced by Edman degradation. The peptides display structural and pharmacological diversity and share little sequence similarity with peptides from other animal venoms, which indicates the venom of O. huwena Wang is unique. The venom peptides can be classified into several superfamilies. Also it is revealed that gene duplication and focal hypermutation have taken place during the evolution of the spider toxins.
虎纹捕鸟蛛（Ornithoctonus huwena Wang）是中国一种毒性很强的蜘蛛。此前已从这种蜘蛛的毒液中鉴定出几种具有不同生物活性类型的化合物。在本研究中，我们对该毒液进行了蛋白质组学和肽组学分析。毒液被预先分离为两部分：分子量（MW）高于10,000的毒液蛋白和MW低于10,000的毒液肽。使用一维凝胶电泳（1-DE）、二维凝胶电泳（2-DE）和质谱法，鉴定出90种蛋白质，包括一些重要的酶、结合蛋白以及一些具有重要生物学功能的蛋白质。对于毒液肽，采用了阳离子交换和反相色谱相结合的方法。通过质谱检测到100多个组分，通过埃德曼降解法对47种肽进行了测序。这些肽表现出结构和药理学多样性，并且与其他动物毒液中的肽序列相似性很低，这表明虎纹捕鸟蛛的毒液是独特的。毒液肽可分为几个超家族。此外还揭示出在蜘蛛毒素的进化过程中发生了基因复制和局部超突变。
