School of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Feb;27(2):255-61. doi: 10.1080/19440040903314015.
Deoxynivalenol (DON) is a toxic fungal metabolite that frequently contaminates cereal crops including wheat, maize and barley. Despite knowledge of frequent exposure through diet, our understanding of the potential consequences of human exposure remains limited, in part due to the lack of validated exposure biomarkers. In this study, we interrogated the urinary metabolome using nuclear magnetic resonance (NMR) spectroscopy to compare individuals with known low and high DON exposure through consumption of their normal diet. Urine samples from 22 adults from the UK (seven males, 15 females; age range = 21-59 years) had previously determined urinary DON levels using an established liquid chromatography-mass spectrometry (LC-MS) assay. Urine samples were subsequently analysed using an NMR-based metabolomics approach coupled with multivariate statistical analysis. Metabolic profiling suggested that hippurate levels could be used to distinguish between groups with low (3.6 ng DON mg(-1) creatinine: 95% CI = 2.6, 5.0 ng mg(-1)) and high (11.1 ng mg(-1): 95% CI = 8.1, 15.5 ng mg(-1)) DON exposure, with the concentration of hippurate being significantly (1.5 times) higher for people with high DON exposure than for those with low DON exposure (p = 0.047). This, to our knowledge, is the first report of a metabolomics-derived biomarker of DON exposure in humans.
脱氧雪腐镰刀菌烯醇（DON）是一种有毒的真菌代谢物，经常污染包括小麦、玉米和大麦在内的谷物作物。尽管人们通过饮食频繁接触到 DON，但我们对人类接触的潜在后果的了解仍然有限，部分原因是缺乏经过验证的暴露生物标志物。在这项研究中，我们使用核磁共振（NMR）光谱技术对尿液代谢组进行了检测，以比较通过正常饮食摄入已知低和高 DON 暴露个体的情况。英国的 22 名成年人（7 名男性，15 名女性；年龄范围=21-59 岁）的尿液样本先前已使用经过验证的液相色谱-质谱（LC-MS）测定法确定了尿液中的 DON 水平。随后，使用基于 NMR 的代谢组学方法结合多元统计分析对尿液样本进行了分析。代谢谱分析表明，马尿酸水平可用于区分低 DON 暴露组（3.6 ng DON mg(-1) 肌酐：95%CI=2.6，5.0 ng mg(-1))和高 DON 暴露组（11.1 ng mg(-1)：95%CI=8.1，15.5 ng mg(-1)），高 DON 暴露者的马尿酸浓度明显（1.5 倍）高于低 DON 暴露者（p=0.047）。据我们所知，这是人类 DON 暴露的代谢组学衍生生物标志物的首次报道。
