法国诺曼底男性农民尿液中脱氧雪腐镰刀菌烯醇和脱环氧脱氧雪腐镰刀菌烯醇的决定因素。

Determinants of urinary deoxynivalenol and de-epoxy deoxynivalenol in male farmers from Normandy, France.

机构信息

Molecular Epidemiology Unit, Centre for Epidemiology and Biostatistics, Leeds Institute of Genetics, Health and Therapeutics.

出版信息

J Agric Food Chem. 2010 Apr 28;58(8):5206-12. doi: 10.1021/jf100892v.

DOI:10.1021/jf100892v

PMID:20349912

Abstract

Dietary exposure to deoxynivalenol (DON) from contaminated cereal crops is frequent in Europe, and farm workers who handle grain or silage may be at additional risk. In this study we refined a urinary assay for DON and present a novel assay for the DON metabolite de-epoxy-deoxynivalenol (DOM-1). These were applied to a pilot survey of male French farmers (n = 76, aged 23-74). DON was detected in 75/76 samples (range 0.5-28.8 ng/mL) and DOM-1 in 26/76 samples (range 0.2-2.8 ng/mL). In multivariate analysis including creatinine as a covariate, bread consumption, other cereal consumption, and maize acreage contributed to the model, explaining the variation in urinary "DON and DOM-1" concentration combined (R(2) = 0.33). This is the first exposure biomarker survey for DON in a French population, and the first demonstration of urinary DOM-1 in humans. Further investigations into occupational activity, handling, or airborne exposures would be informative.

摘要

在欧洲，受污染谷物作物导致的膳食脱氧雪腐镰刀菌烯醇（DON）暴露较为频繁，接触谷物或青贮饲料的农场工人可能面临更大的风险。本研究对尿液 DON 检测方法进行了改进，并建立了 DON 代谢物去环氧脱氧雪腐镰刀菌烯醇（DOM-1）的新型检测方法。我们将这些方法应用于法国男性农民的一项试点调查（n = 76，年龄 23-74 岁）。76 个样本中均检出 DON（范围 0.5-28.8ng/mL），26 个样本中检出 DOM-1（范围 0.2-2.8ng/mL）。在包含肌酐作为协变量的多变量分析中，面包摄入、其他谷物摄入和玉米种植面积均对模型有贡献，解释了尿液中“DON 和 DOM-1”浓度的联合变化（R²=0.33）。这是法国人群中首次对 DON 进行暴露生物标志物调查，也是首次在人体中检测到尿液 DOM-1。进一步调查职业活动、处理或空气传播暴露情况将提供有价值的信息。