Sarkanj Bojan, Warth Benedikt, Uhlig Silvio, Abia Wilfred A, Sulyok Michael, Klapec Tomislav, Krska Rudolf, Banjari Ines
Subdepartment of Biochemistry and Toxicology, Department of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University, Osijek, Croatia; Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Austria.
Food Chem Toxicol. 2013 Dec;62:231-7. doi: 10.1016/j.fct.2013.08.043. Epub 2013 Aug 28.
In this pilot survey the levels of various mycotoxin biomarkers were determined in third trimester pregnant women from eastern Croatia. First void urine samples were collected and analysed using a "dilute and shoot" LC-ESI-MS/MS multi biomarker method. Deoxynivalenol (DON) and its metabolites: deoxynivalenol-15-glucuronide and deoxynivalenol-3-glucuronide were detected in 97.5% of the studied samples, partly at exceptionally high levels, while ochratoxin A was found in 10% of the samples. DON exposure was primarily reflected by the presence of deoxynivalenol-15-glucuronide with a mean concentration of 120 μg L(-1), while free DON was detected with a mean concentration of 18.3 μg L(-1). Several highly contaminated urine samples contained a third DON conjugate, tentatively identified as deoxynivalenol-7-glucuronide by MS/MS scans. The levels of urinary DON and its metabolites measured in this study are the highest ever reported, and 48% of subjects were estimated to exceed the provisional maximum tolerable daily intake (1 μg kg(-1) b.w.).
在这项初步调查中，对来自克罗地亚东部的孕晚期妇女体内各种霉菌毒素生物标志物的水平进行了测定。收集首次晨尿样本，并使用“稀释进样”液相色谱 - 电喷雾串联质谱多生物标志物方法进行分析。在97.5%的研究样本中检测到脱氧雪腐镰刀菌烯醇（DON）及其代谢产物：脱氧雪腐镰刀菌烯醇 - 15 - 葡萄糖醛酸苷和脱氧雪腐镰刀菌烯醇 - 3 - 葡萄糖醛酸苷，部分样本中的含量异常高，而在10%的样本中发现了赭曲霉毒素A。DON暴露主要通过脱氧雪腐镰刀菌烯醇 - 15 - 葡萄糖醛酸苷的存在来反映，其平均浓度为120μg L⁻¹，而游离DON的检测平均浓度为18.3μg L⁻¹。几个高度污染的尿液样本中含有第三种DON共轭物，通过串联质谱扫描初步鉴定为脱氧雪腐镰刀菌烯醇 - 7 - 葡萄糖醛酸苷。本研究中测得的尿中DON及其代谢产物水平是有史以来报道的最高水平，估计48%的受试者超过了暂定每日最大耐受摄入量（1μg kg⁻¹体重）。
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