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cDNA and deduced amino acid sequences of a dog liver cytochrome P-450 of the IIIA gene subfamily.

作者信息

Ciaccio P J, Graves P E, Bourque D P, Glinsmann-Gibson B, Halpert J R

机构信息

Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, PA 19111.

出版信息

Biochim Biophys Acta. 1991 Feb 16;1088(2):319-22. doi: 10.1016/0167-4781(91)90072-t.

DOI:10.1016/0167-4781(91)90072-t
PMID:2001406
Abstract

A 1.96 kbp cDNA encoding a male Beagle dog liver cytochrome P-450 of 503 amino acid residues (Mr 57,636) has been isolated and sequenced. The deduced amino acid sequence is 79.8%, 69.3% and 74.1% identical to the P450IIIA forms human NF25, rat PCN1 and rabbit LM3c, respectively. The amino terminal sequence is identical to the first 28 residues of the dog P450IIIA form PBD-1. Southern blot analysis yields restriction patterns consistent with IIIA gene subfamily multiplicity.

摘要

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