Feller G, Thiry M, Gerday C
Laboratory of Biochemistry, University of Liège, Belgium.
Biochim Biophys Acta. 1991 Feb 16;1088(2):323-4. doi: 10.1016/0167-4781(91)90073-u.
A lipase gene (lip3) from the psychotrophic strain Moraxella TA144 has been cloned and sequenced. The deduced primary structure of the lipase preprotein is composed of 315 amino acids with a predicted Mr of 34,772. This enzyme contains two consensus peptides showing cluster of glycine residues that may be involved in domain flexibility. The cloned gene product conserves the low temperature activity and the thermolability properties of the wild enzyme.
已克隆并测序了嗜冷菌株莫拉克斯氏菌TA144的一个脂肪酶基因（lip3）。推导的脂肪酶前体蛋白一级结构由315个氨基酸组成，预测分子量为34772。该酶含有两个共有肽段，显示出可能与结构域灵活性有关的甘氨酸残基簇。克隆的基因产物保留了野生型酶的低温活性和热不稳定特性。
