Arpigny J L, Feller G, Gerday C
Laboratoire de Biochimie, Université de Liège-Sart Tilman, Belgium.
Biochim Biophys Acta. 1993 Jan 23;1171(3):331-3. doi: 10.1016/0167-4781(93)90078-r.
A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold.