Chilian W M, DeFily D V
Department of Medical Physiology, Texas A & M University College of Medicine, College Station.
Blood Vessels. 1991;28(1-3):236-44. doi: 10.1159/000158868.
Measurements of coronary microvascular parameters in situ are difficult because of the thickness of the heart muscle and cardiac contraction. Both of these problems hamper the visualization of the coronary microcirculation. We have refined methodological approaches that enable the study of the coronary microcirculation in situ. In the first approach, microvessels can be visualized in the beating heart using a preparation that compensates for cardiac motion by creating an illusion that the heart is motionless. This is accomplished by flashing a stroboscopic light source once per heart cycle at the same point in each cycle and synchronizing a ventilator with the cardiac cycle. Images of microvessels can be obtained using standard intravital video-microscopic techniques. To visualize the intramural and subendocardial microcirculation, studies are completed in isolated hearts. In this preparation, measurements of microvascular diameters and pressures can be performed in both the subepicardial and subendocardial microcirculations. This latter approach allows insight into transmural differences of coronary microvascular regulation.
由于心肌厚度和心脏收缩，原位测量冠状动脉微血管参数具有一定难度。这两个问题都妨碍了冠状动脉微循环的可视化。我们改进了方法，能够在原位研究冠状动脉微循环。第一种方法是，通过一种能补偿心脏运动的制备方法，在跳动的心脏中使微血管可视化，该方法通过在每个心动周期的同一点处频闪一次光源，并使呼吸机与心动周期同步，营造出心脏静止不动的假象。微血管图像可使用标准的活体视频显微镜技术获取。为了可视化壁内和心内膜下微循环，研究在离体心脏中完成。在此制备方法中，可以在心肌外膜下和心内膜下微循环中测量微血管直径和压力。后一种方法有助于深入了解冠状动脉微血管调节的透壁差异。
