Plant Products and Human Nutrition Group, Graham Kerr Building, Division of Ecology and Evolutionary Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK.
J Agric Food Chem. 2010 Apr 14;58(7):3910-5. doi: 10.1021/jf902267v.
Anthocyanins in extracts from raspberries and blueberries were analyzed by reversed-phase HPLC coupled to a high-resolution Exactive Orbitrap mass spectrometer (HR-MS) with a resolution of 100,000, operated with an electrospray source in the positive ionization mode. As consumption of anthocyanin-rich berry extracts has been associated with improved cognitive function, brain extracts from European greenfinches ( Carduelis chloris ) that had been fed one blackberry daily for a period of 2 weeks were analyzed by both HPLC with traditional tandem MS in the selected reaction monitoring mode and HPLC-HR-MS. Cyanidin-3-O-glucoside was detected in the brain extracts by both methods, but because of its high level of selectivity, HR-MS was ca. 200-fold more sensitive. A further advantage of HR-MS is that unlike MS-SRM it enables both targeted and nontargeted compounds to be detected and much lower limits of detection are achieved without compromising the selectivity of the analysis.
采用反相高效液相色谱法（RP-HPLC）与高分辨率 Exactive Orbitrap 质谱联用（HR-MS），对树莓和蓝莓提取物中的花色苷进行分析，HR-MS 的分辨率为 100,000，采用电喷雾源，正离子模式下运行。由于消费富含花色苷的浆果提取物与改善认知功能有关，因此对连续 2 周每天喂食黑莓的欧洲金翅雀（Carduelis chloris）的脑提取物进行了分析，采用传统串联 MS 的 HPLC 以及 HPLC-HR-MS 进行分析。两种方法均在脑提取物中检测到矢车菊素-3-O-葡萄糖苷，但由于其高选择性，HR-MS 的灵敏度约高 200 倍。与 MS-SRM 不同，HR-MS 的另一个优点是，它既能检测到靶向化合物，也能检测到非靶向化合物，在不影响分析选择性的情况下，还能实现更低的检测限。
