锦葵中花色苷的高效薄层色谱法和反射模式密度测定法：与梯度洗脱反相高效液相色谱法的比较

HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC.

作者信息

Farina A, Doldo A, Cotichini V, Rajevic M, Quaglia M G, Mulinacci N, Vincieri F F

机构信息

Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanità, Rome, Italy.

出版信息

J Pharm Biomed Anal. 1995 Dec;14(1-2):203-11. doi: 10.1016/0731-7085(95)01632-5.

DOI:10.1016/0731-7085(95)01632-5

PMID:8833983

Abstract

Aqueous alcoholic mallow flower extracts were analyzed both by HPTLC-densitometry in the reflectance mode at 530 nm and by reversed-phase HPLC with gradient elution. For the mallow flower anthocyanins the best chromatographic resolution was obtained by HPLC, which revealed only two main compounds, confirmed by FAB-MS: malvidin 3,5-O-diglucoside (malvin) and malvidin 3-O-(6"-O-malonylglucoside)-5-O-glucoside. The HPTLC densitometric method on cellulose plates provides accuracy, reproducibility and selectivity for the quantitative analysis of the anthocyanins and this method was shown to be much more sensitive than the HPLC-DAD system, at 530 nm. Both methods give comparable quantitative results for total anthocyanins when applied to mallow flowers from two different sources: Italy and Albania.

摘要

采用HPTLC - 密度分析法在530 nm反射模式下以及反相HPLC梯度洗脱法对水醇性锦葵花提取物进行了分析。对于锦葵花花青素，HPLC获得了最佳的色谱分辨率，该方法仅揭示了两种主要化合物，经FAB - MS确认：矢车菊素3,5 - O - 二葡萄糖苷（锦葵色素）和矢车菊素3 - O -（6“ - O - 丙二酰葡萄糖苷）- 5 - O - 葡萄糖苷。纤维素板上的HPTLC密度分析法为花青素的定量分析提供了准确性、重现性和选择性，并且该方法在530 nm处比HPLC - DAD系统灵敏得多。当应用于来自意大利和阿尔巴尼亚两个不同来源的锦葵花时，两种方法对总花青素给出了可比的定量结果。