Department of Chemistry, University of California, Irvine, California 92697, USA.
ACS Nano. 2010 Jan 26;4(1):199-204. doi: 10.1021/nn901256s.
Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide have been prepared by inverse microemulsion polymerization. Peptide affinity was achieved in part by incorporating the target (imprint) peptide in the polymerization reaction mixture. Incorporation of the imprint peptide assists in the creation of complementary binding sites in the resulting polymer nanoparticle (NP). To orient the imprint peptide at the interface of the water and oil domains during polymerization, the peptide target was coupled with fatty acid chains of varying length. The peptide--NP binding affinities (ca. 90-900 nM) were quantitatively evaluated by a quartz crystal microbalance (QCM). The optimal chain length was established that created high affinity peptide binding sites on the surface of the nanoparticles. This method can be used for the preparation of nanosized synthetic polymers with antibody-like affinity for hydrophilic peptides and proteins ("plastic antibodies").
通过反相微乳液聚合制备了对亲水性肽具有抗体样亲和力的合成聚合物纳米粒子。通过将目标（印迹）肽掺入聚合反应混合物中，部分实现了肽亲和力。印迹肽的掺入有助于在所得聚合物纳米粒子（NP）中形成互补结合位点。为了在聚合过程中将印迹肽定向在水和油相的界面处，将肽靶与不同长度的脂肪酸链偶联。通过石英晶体微天平（QCM）定量评估肽-NP 结合亲和力（约 90-900 nM）。确定了最佳链长，在纳米粒子表面上形成了高亲和力的肽结合位点。该方法可用于制备对亲水性肽和蛋白质具有抗体样亲和力的纳米级合成聚合物（“塑料抗体”）。
