Department of Molecular Genetics, Institute of Pathology, University of Ljubljana, Slovenia.
Cancer Invest. 2010 Jun;28(5):487-94. doi: 10.3109/07357900903476752.
The purpose of this study was to find novel gene(s) involved in the development of lung adenocarcinoma (AD). Using DNA microarrays, we identified 31 up-regulated and 8 downregulated genes in 12 AD. Real time PCR was used to measure expression of VIPR1 and SPP1 mRNA and possible losses or gains of genes in 32 AD. We describe significant upregulation of the SPP1 gene, downregulation of VIPR1, and losses of the VIPR1 gene. Our findings complement a proposed VIPR1 tumor suppressor role, in which deletions in the 3p22 chromosome region are an important mechanism leading to loss of the VIPR1 gene.
本研究旨在寻找参与肺腺癌（AD）发生的新基因。通过 DNA 微阵列，我们在 12 例 AD 中鉴定出 31 个上调和 8 个下调基因。实时 PCR 用于测量 32 例 AD 中 VIPR1 和 SPP1 mRNA 的表达和基因的可能缺失或获得。我们描述了 SPP1 基因的显著上调、VIPR1 的下调以及 VIPR1 基因的缺失。我们的研究结果补充了 VIPR1 肿瘤抑制因子作用的假说，其中 3p22 染色体区域的缺失是导致 VIPR1 基因缺失的重要机制。
