Department of Thoracic Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jiefang Road, Shangcheng District, Hangzhou, 310009, China.
Med Oncol. 2019 Sep 27;36(11):91. doi: 10.1007/s12032-019-1312-y.
The vasoactive intestinal peptide receptor-1(VIPR1) has prominent growth effects on a number of common neoplasms. However, there were contradictions in the effect cross different cancers. We aimed to explore the effect of VIPR1 overexpression on a human lung adenocarcinoma cell line H1299. GEO dataset was used to screen differentially expressed genes in lung adenocarcinoma tissues. The expression of VIPR1 mRNA was determined in the cancer Genome Atlas (TCGA). Immunohistochemical analysis was performed to determine VIPR1 protein expression in lung adenocarcinoma and corresponding adjacent tissues (n = 22). Fluorescence real-time quantitative PCR detected the expression of VIPR1 in human normal lung epithelial cell line BEAS-2B and lung adenocarcinoma cell line H1299. Overexpression strategies were employed to assess functions of VIPR1 expression on several malignant phenotypes in H1299. The expression of VIPR1 was lower in lung adenocarcinoma tissues than that in adjacent tissues. Compared with the normal lung epithelial cells BEAS-2B, VIPR1 was down-regulated in lung cancer cells H1299 (P < 0.05). After the overexpression of VIPR1, we found that VIPR1 significantly inhibited growth, migration, and invasion of H1299 cells (P < 0.05). Our findings point out the tumor suppressor roles of VIPR1 in human LUAD pathogenesis.
血管活性肠肽受体-1(VIPR1)对许多常见肿瘤具有显著的生长作用。然而，不同癌症之间的作用存在矛盾。我们旨在探索 VIPR1 过表达对人肺腺癌细胞系 H1299 的影响。GEO 数据集用于筛选肺腺癌组织中差异表达的基因。癌症基因组图谱(TCGA)中确定了 VIPR1 mRNA 的表达。免疫组织化学分析用于确定肺腺癌和相应的相邻组织中 VIPR1 蛋白的表达(n=22)。荧光实时定量 PCR 检测人正常肺上皮细胞系 BEAS-2B 和肺腺癌细胞系 H1299 中 VIPR1 的表达。采用过表达策略来评估 VIPR1 表达对 H1299 中几种恶性表型的功能。肺腺癌组织中 VIPR1 的表达低于相邻组织。与正常肺上皮细胞 BEAS-2B 相比，肺癌细胞 H1299 中 VIPR1 下调(P<0.05)。过表达 VIPR1 后，我们发现 VIPR1 显著抑制 H1299 细胞的生长、迁移和侵袭(P<0.05)。我们的研究结果指出 VIPR1 在人类 LUAD 发病机制中起肿瘤抑制作用。
