Cranfield Health, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK.
J Chromatogr A. 2010 Apr 16;1217(16):2543-7. doi: 10.1016/j.chroma.2009.11.091. Epub 2009 Dec 3.
Two polymers were computationally designed with affinity to two of the most abundant mycotoxins aflatoxin B1 (AFB1) and ochratoxin A (OTA) for application in the ToxiQuant T1 System. The principle of quantification of AFB1 and OTA using the ToxiQuant T1 instrument comprised of a fluorimetric analysis of mycotoxins adsorbed on the polymer upon exposure to UV light. High affinity of the developed resins allowed the adsorption of both toxins as discrete bands on the top of the cartridge with detection limit as low as 1ng quantity of mycotoxins.
两种聚合物经过计算设计，对两种最丰富的霉菌毒素黄曲霉毒素 B1（AFB1）和赭曲霉毒素 A（OTA）具有亲和力，可用于 ToxiQuant T1 系统。使用 ToxiQuant T1 仪器定量检测 AFB1 和 OTA 的原理包括在暴露于紫外光下时，对吸附在聚合物上的霉菌毒素进行荧光分析。开发的树脂具有高亲和力，允许两种毒素作为离散带吸附在试剂盒的顶部，检测限低至 1ng 霉菌毒素。
