Giovannoli Cristina, Spano Giulia, Di Nardo Fabio, Anfossi Laura, Baggiani Claudio
Laboratory of Bioanalytical Chemistry, Department of Chemistry, University of Torino, Via Giuria 5, Torino 10125, Italy.
Toxins (Basel). 2017 May 20;9(5):174. doi: 10.3390/toxins9050174.
Patulin is a water-soluble mycotoxin produced by several species of fungi. Governmental bodies have placed it under scrutiny for its potential negative health effects, and maximum residue limits are fixed in specific food matrices to protect consumers' health. Confirmatory analysis of patulin in complex food matrices can be a difficult task, and sample clean-up treatments are frequently necessary before instrumental analyses. With the aim of simplifying the clean-up step, we prepared a 256-member combinatorial polymeric library based on 16 functional monomers, four cross-linkers and four different porogenic solvents. The library was screened for the binding towards patulin in different media (acetonitrile and citrate buffer at pH 3.2), with the goal of identifying polymer formulations with good binding properties towards the target compound. As a proof of concept, a methacrylic acid-co-pentaerithrytole tetraacrylate polymer prepared in chloroform was successfully used as a solid-phase extraction material for the clean-up and extraction of patulin from apple juice. Clean chromatographic patterns and acceptable recoveries were obtained for juice spiked with patulin at concentration levels of 25 (64 ± 12%), 50 (83 ± 5.6%) and 100 μg L (76 ± 4.5%). The within-day and between-day reproducibility evaluated at a concentration level of 25 μg L were 5.6 and 7.6%, respectively.
展青霉素是由几种真菌产生的一种水溶性霉菌毒素。政府机构因其潜在的负面健康影响而对其进行审查，并在特定食品基质中设定了最大残留限量以保护消费者健康。在复杂食品基质中对展青霉素进行确证分析可能是一项艰巨的任务，并且在仪器分析之前通常需要进行样品净化处理。为了简化净化步骤，我们基于16种功能单体、4种交联剂和4种不同的致孔溶剂制备了一个包含256个成员的组合聚合物库。对该库在不同介质（乙腈和pH 3.2的柠檬酸盐缓冲液）中与展青霉素的结合情况进行筛选，目的是鉴定出对目标化合物具有良好结合性能的聚合物配方。作为概念验证，在氯仿中制备的甲基丙烯酸 - 季戊四醇四丙烯酸酯共聚物成功用作固相萃取材料，用于从苹果汁中净化和萃取展青霉素。对于添加了浓度为25（64±12%）、50（83±5.6%）和100 μg/L（76±4.5%）展青霉素的果汁，获得了干净的色谱图和可接受的回收率。在25 μg/L浓度水平下评估的日内和日间重现性分别为5.6%和7.6%。
