Department of Medicine, Shiga University of Medical Science, Shiga, Japan.
Obesity (Silver Spring). 2010 Jul;18(7):1277-82. doi: 10.1038/oby.2009.442. Epub 2009 Dec 17.
To determine the potential role of the transcriptional factor-activating enhancer-binding protein-2beta (TFAP2B) in the regulation of expression of adipokines, adiponectin, leptin, and interleukin-6 (IL-6) in vivo, we quantified the mRNA expression levels of these adipokines and TFAP2B in visceral (omental) and abdominal subcutaneous adipose tissues of 66 individuals with variable degree of adiposity and studied their correlations with BMI and their plasma concentrations. We found that BMI correlated negatively with plasma adiponectin levels and positively with those of leptin. Adiponection mRNA expression in subcutaneous fat correlated negatively with BMI, whereas leptin mRNA levels in the omentum correlated with plasma leptin levels and BMI. In contrast, IL-6 mRNA levels in subcutaneous and omental fat did not correlate with BMI. IL-6 mRNA levels in the omental fat correlated with plasma IL-6 levels. Whereas TFAP2B mRNA expression did not correlate with BMI, it correlated negatively with adiponectin expression in the subcutaneous adipose tissue. Furthermore, TFAP2B mRNA expression correlated negatively with leptin and positively with IL-6 expression in both subcutaneous and omental adipose tissues. These relationships are consistent with our in vitro observations and indicate that TFAP2B seems to regulate the expression of various adipokines in vivo.
为了确定转录因子激活增强子结合蛋白-2β（TFAP2B）在体内调节脂肪因子（脂联素、瘦素和白细胞介素-6（IL-6））表达中的潜在作用，我们定量检测了 66 名个体内脏（网膜）和腹部皮下脂肪组织中这些脂肪因子和 TFAP2B 的 mRNA 表达水平，并研究了它们与 BMI 和血浆浓度的相关性。我们发现 BMI 与血浆脂联素水平呈负相关，与瘦素水平呈正相关。皮下脂肪中脂联素 mRNA 表达与 BMI 呈负相关，而网膜中瘦素 mRNA 水平与血浆瘦素水平和 BMI 呈正相关。相比之下，皮下和网膜脂肪中的 IL-6 mRNA 水平与 BMI 无关。网膜脂肪中的 IL-6 mRNA 水平与血浆 IL-6 水平相关。尽管 TFAP2B mRNA 表达与 BMI 无关，但与皮下脂肪中的脂联素表达呈负相关。此外，TFAP2B mRNA 表达与皮下和网膜脂肪中的瘦素和 IL-6 表达呈正相关。这些关系与我们的体外观察结果一致，表明 TFAP2B 似乎在体内调节各种脂肪因子的表达。
