代表衣原体属特异性脂多糖表位的人工糖缀合物的结构、血清学特异性及合成
Structure, serological specificity, and synthesis of artificial glycoconjugates representing the genus-specific lipopolysaccharide epitope of Chlamydia spp.
作者信息
Holst O, Brade L, Kosma P, Brade H
机构信息
Division of Biochemical Microbiology, Institut für Experimentelle Biologie und Medizin, Forschungsinstitut Borstel, Federal Republic of Germany.
出版信息
J Bacteriol. 1991 Mar;173(6):1862-6. doi: 10.1128/jb.173.6.1862-1866.1991.
Abstract
The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta 1-6-GlcNol (Kdo, 3-deoxy-D-manno-2-octulosonic acid). Immunochemical studies on this pentasaccharide and the chemically synthesized partial structures Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta, Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha, Kdop alpha 2-4-Kdop alpha, Kdop alpha 2-8-Kdop alpha, and Kdop alpha using artificial glycoconjugate antigens and monoclonal antibodies showed that fatty acids and phosphoryl groups (as present in native lipopolysaccharide) are dispensable for constitution of the genus-specific epitope and that the minimal structure to exhibit chlamydia specificity is the Kdo trisaccharide moiety.
摘要
人类细菌病原体衣原体属拥有一种属特异性脂多糖作为主要表面抗原，其结构已通过分析化学确定为Kdoα2-8-Kdoα2-4-Kdoα2-6GlcNpβ1-6-GlcNol（Kdo，3-脱氧-D-甘露-2-辛酮糖酸）。使用人工糖缀合物抗原和单克隆抗体对该五糖以及化学合成的部分结构Kdoα2-8-Kdoα2-4-Kdoα2-6GlcNpβ、Kdoα2-8-Kdoα2-4-Kdoα Kdoα2-4-Kdoα、Kdoα2-8-Kdoα和Kdoα进行的免疫化学研究表明，脂肪酸和磷酸基团（天然脂多糖中存在）对于属特异性表位的构成是可有可无的，并且表现出衣原体特异性的最小结构是Kdo三糖部分。
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