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沙眼衣原体、沙门氏菌属Re突变体及醋酸钙不动杆菌无硝变种中与免疫相关的含酮脱氧辛糖酸结构。

Immunologically related ketodeoxyoctonate-containing structures in Chlamydia trachomatis, Re mutants of Salmonella species, and Acinetobacter calcoaceticus var. anitratus.

作者信息

Nurminen M, Wahlström E, Kleemola M, Leinonen M, Saikku P, Mäkelä P H

出版信息

Infect Immun. 1984 Jun;44(3):609-13. doi: 10.1128/iai.44.3.609-613.1984.

DOI:10.1128/iai.44.3.609-613.1984
PMID:6427112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263641/
Abstract

The lipopolysaccharides (LPS) of Chlamydia trachomatis, Acinetobacter calcoaceticus var. anitratus, and Re mutants of Salmonella sp. were shown to share related immunodeterminants , as demonstrated by double immunodiffusion and immunoblotting from sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. The cross-reactive material in the extracellular slime of A. calcoaceticus var. anitratus was shown to be released LPS. The Acinetobacter LPS was found to separate in sodium dodecyl sulfate-polyacrylamide gel electrophoresis into three fractions. The cross-reactive component was the fraction migrating fastest, at a rate identical to Re-type LPS of Salmonella sp. The Acinetobacter LPS could be used as antigen in complement fixation assays performed on paired sera of patients with chlamydial pneumonia; it gave results identical to those of the chlamydial complement fixation glycolipid antigen conventionally used in such assays in 9 of 10 patients.

摘要

沙眼衣原体、醋酸钙不动杆菌无硝变种及沙门氏菌Re突变株的脂多糖经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶的双向免疫扩散和免疫印迹显示,具有相关免疫决定簇。醋酸钙不动杆菌无硝变种胞外黏液中的交叉反应物质被证明是释放的脂多糖。不动杆菌脂多糖在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中可分离成三个组分。交叉反应成分是迁移最快的组分,其迁移速率与沙门氏菌的Re型脂多糖相同。不动杆菌脂多糖可用作衣原体肺炎患者配对血清补体结合试验的抗原;在10例患者中有9例,其结果与此类试验中常规使用的衣原体补体结合糖脂抗原的结果相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad47/263641/f06fe2baccd5/iai00129-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad47/263641/319340909fc9/iai00129-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad47/263641/79cd8dd74e92/iai00129-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad47/263641/f06fe2baccd5/iai00129-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad47/263641/319340909fc9/iai00129-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad47/263641/79cd8dd74e92/iai00129-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad47/263641/f06fe2baccd5/iai00129-0076-a.jpg

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