衣原体属特异性脂多糖表位的化学与血清学研究
Chemical and serological investigations on the genus-specific lipopolysaccharide epitope of Chlamydia.
作者信息
Brade H, Brade L, Nano F E
出版信息
Proc Natl Acad Sci U S A. 1987 Apr;84(8):2508-12. doi: 10.1073/pnas.84.8.2508.
Abstract
Members of the bacterial genus Chlamydia are responsible for widespread disease among humans and animals, including endemic trachoma in developing countries, venereal disease in developed countries, and a variety of other diseases such as infantile pneumonia and lymphogranuloma venereum. Although there is little genetic relatedness between and large antigenic diversity between and among the two chlamydial species, one antigenic determinant has been preserved among all serovars: the genus-specific lipopolysaccharide epitope. In this report, the tools of molecular genetics, monoclonal antibodies, and analytical and synthetic chemistry have been combined to determine the structure of this epitope. This epitope is attributed to the presence of a trisaccharide of 3-deoxy-D-manno-octulosonic acid (KDO) of the sequence KDOp-(2----8)-KDOp-(2----4)-KDO. The structure includes a unique linkage of two KDO residues through a 2.8-linkage.
摘要
衣原体属细菌会引发人类和动物中的广泛疾病，包括发展中国家的地方性沙眼、发达国家的性病，以及多种其他疾病，如婴儿肺炎和性病性淋巴肉芽肿。尽管两种衣原体物种之间几乎没有遗传相关性且抗原多样性很大，但所有血清型中都保留了一个抗原决定簇：属特异性脂多糖表位。在本报告中，分子遗传学工具、单克隆抗体以及分析化学和合成化学方法已被结合起来以确定该表位的结构。该表位归因于存在序列为KDOp-(2→8)-KDOp-(2→4)-KDO的3-脱氧-D-甘露糖辛酮糖酸（KDO）三糖。该结构包括两个KDO残基通过2→8连接的独特连接方式。
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