从表达衣原体脂多糖属特异性表位的大肠杆菌F515重组菌株（Re化学型）的脂多糖中分离出的两种寡糖二磷酸酯的结构分析。
Structural analysis of two oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant strain of Escherichia coli F515 (Re chemotype) expressing the genus-specific epitope of Chlamydia lipopolysaccharide.
作者信息
Holst O, Broer W, Thomas-Oates J E, Mamat U, Brade H
机构信息
Division of Biochemical Microbiology, Forschungsinstitut Borstel, Germany.
出版信息
Eur J Biochem. 1993 Jun 15;214(3):703-10. doi: 10.1111/j.1432-1033.1993.tb17971.x.
The lipopolysaccharide of the recombinant strain Escherichia coli F515-207, expressing the genus-specific epitope of Chlamydia lipopolysaccharide, was sequentially de-O- and de-N-acylated by mild hydrazinolysis and treatment with 4 M KOH, respectively, yielding two oligosaccharide bisphosphates which were isolated by high-performance anion-exchange chromatography and gel-permeation chromatography. Their structures were determined by chemical analysis, NMR spectroscopy, and mass spectrometry as alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha-D-GlcN 1,4'-P2 (tetrasaccharide bisphosphate) and alpha-Kdo-(2-8)-alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha- D-GlcN 1,4'-P2 (pentasaccharide bisphosphate).
表达衣原体脂多糖属特异性表位的重组菌株大肠杆菌F515 - 207的脂多糖，分别通过温和的肼解和用4 M KOH处理依次进行O - 去酰化和N - 去酰化，得到两种寡糖二磷酸酯，通过高效阴离子交换色谱和凝胶渗透色谱分离。通过化学分析、核磁共振光谱和质谱确定它们的结构为α - Kdo - (2 - 4) - α - Kdo - (2 - 6) - β - D - GlcN - (1 - 6) - α - D - GlcN 1,4'-P2（四糖二磷酸酯）和α - Kdo - (2 - 8) - α - Kdo - (2 - 4) - α - Kdo - (2 - 6) - β - D - GlcN - (1 - 6) - α - D - GlcN 1,4'-P2（五糖二磷酸酯）。
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