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大肠杆菌外膜蛋白LamB中麦芽糊精结合位点的化学计量学

Stoichiometry of maltodextrin-binding sites in LamB, an outer membrane protein from Escherichia coli.

作者信息

Gehring K, Cheng C H, Nikaido H, Jap B K

机构信息

Cell and Molecular Biology, Lawrence Berkeley Laboratory, California.

出版信息

J Bacteriol. 1991 Mar;173(6):1873-8. doi: 10.1128/jb.173.6.1873-1878.1991.

DOI:10.1128/jb.173.6.1873-1878.1991
PMID:2001992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207716/
Abstract

We have directly measured the stoichiometry of maltodextrin-binding sites in LamB. Scatchard plots and computer fitting of flow dialysis (rate-of-dialysis) experiments clearly establish three independent binding sites per LamB trimer, with a dissociation constant of approximately 60 microM for maltoheptaose. The current model for LamB's function as a specific pore is discussed with respect to the symmetry in LamB's kinetic properties and the implications of our results.

摘要

我们直接测定了LamB中麦芽糊精结合位点的化学计量。Scatchard图以及流动透析（透析速率）实验的计算机拟合清楚地表明，每个LamB三聚体有三个独立的结合位点，麦芽七糖的解离常数约为60微摩尔。我们根据LamB动力学性质的对称性以及我们结果的含义，讨论了目前关于LamB作为特定孔道功能的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95c1/207716/a06e28294296/jbacter00096-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95c1/207716/a06e28294296/jbacter00096-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95c1/207716/a06e28294296/jbacter00096-0047-a.jpg

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Stoichiometry of maltodextrin-binding sites in LamB, an outer membrane protein from Escherichia coli.大肠杆菌外膜蛋白LamB中麦芽糊精结合位点的化学计量学
J Bacteriol. 1991 Mar;173(6):1873-8. doi: 10.1128/jb.173.6.1873-1878.1991.
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本文引用的文献

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Diffusion of solutes through channels produced by phage lambda receptor protein of Escherichia coli: inhibition by higher oligosaccharides of maltose series.溶质通过大肠杆菌噬菌体λ受体蛋白产生的通道的扩散：麦芽糖系列高级寡糖的抑制作用。
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The receptor protein of phage lambda: purification, characterization and preliminary electrical studies in planar lipid bilayers.λ噬菌体的受体蛋白：纯化、表征及在平面脂质双分子层中的初步电学研究
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Noise analysis of ion current through the open and the sugar-induced closed state of the LamB channel of Escherichia coli outer membrane: evaluation of the sugar binding kinetics to the channel interior.通过大肠杆菌外膜LamB通道开放态和糖诱导关闭态的离子电流噪声分析：糖与通道内部结合动力学的评估
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Lambda receptor in the outer membrane of Escherichia coli as a binding protein for maltodextrins and starch polysaccharides.大肠杆菌外膜中的λ受体作为麦芽糊精和淀粉多糖的结合蛋白。
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Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli.大肠杆菌λ噬菌体受体蛋白产生的扩散通道的特异性
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Phage lambda receptor (lamB protein) in Escherichia coli.大肠杆菌中的噬菌体λ受体（LamB蛋白）。
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Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12.λ受体的基因序列，大肠杆菌K12的一种外膜蛋白。
Cell. 1981 Dec;27(3 Pt 2):507-14. doi: 10.1016/0092-8674(81)90392-5.
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Isolation of the bacteriophage lambda receptor from Escherichia coli.从大肠杆菌中分离噬菌体λ受体
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Substrate specificity of the Escherichia coli maltodextrin transport system and its component proteins.大肠杆菌麦芽糊精转运系统及其组成蛋白的底物特异性。
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