热休克调节蛋白RpoH在大肠杆菌碳饥饿蛋白合成及存活中的作用
Role of RpoH, a heat shock regulator protein, in Escherichia coli carbon starvation protein synthesis and survival.
作者信息
Jenkins D E, Auger E A, Matin A
机构信息
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
出版信息
J Bacteriol. 1991 Mar;173(6):1992-6. doi: 10.1128/jb.173.6.1992-1996.1991.
Abstract
Escherichia coli starvation proteins include several heat shock proteins whose induction by heat is controlled by the minor sigma factor, sigma 32. The level of sigma 32 increased in wild-type E. coli upon starvation, and three sigma 32-controlled heat shock proteins (DnaK, GroEL, and HtpG) were not induced during starvation in an isogenic delta rpoH strain, which is unable to synthesize sigma 32. Thus, sigma 32 plays a role in the induction of these proteins during both heat shock and starvation. The delta rpoH strain was more sensitive to starvation but could develop starvation-mediated cross protection against heat and oxidation.
摘要
大肠杆菌饥饿蛋白包括几种热休克蛋白，其受热诱导由次要的σ因子σ32控制。在饥饿条件下，野生型大肠杆菌中σ32的水平升高，而在无法合成σ32的同基因ΔrpoH菌株中，饥饿期间三种受σ32控制的热休克蛋白（DnaK、GroEL和HtpG）未被诱导。因此，σ32在热休克和饥饿期间这些蛋白质的诱导过程中均发挥作用。ΔrpoH菌株对饥饿更敏感，但可产生饥饿介导的对热和氧化的交叉保护作用。
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