Department of Biomedical Sciences, Cancer Research Institute, and Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.
J Proteome Res. 2010 Feb 5;9(2):689-99. doi: 10.1021/pr901013d.
Multiple reaction monitoring was used to verify target proteins in 3 groups of vitreous and plasma samples from 3 stages of diabetic retinopathy: macular hole (nondiabetic control), nonproliferative diabetic retinopathy, and proliferative diabetic retinopathy. Twelve target proteins were quantified using triple quadrupole LC-MS/MS and 3 methods to determine the transitions (information-dependent analysis, the MIDAS workflow, and the PeptideAtlas database). This study might be the first MRM experiment to analyze large numbers of clinical vitreous and plasma samples for biomarker verification. Consequently, several biomarker candidates were identified for use in further applications.
采用多重反应监测技术，在糖尿病视网膜病变的 3 个阶段的玻璃体液和血浆样本的 3 组中验证了靶蛋白：黄斑裂孔（非糖尿病对照）、非增殖性糖尿病视网膜病变和增殖性糖尿病视网膜病变。使用三重四极杆 LC-MS/MS 和 3 种方法（信息依赖分析、MIDAS 工作流程和 PeptideAtlas 数据库）对 12 种靶蛋白进行定量分析。这项研究可能是首次使用 MRM 实验分析大量临床玻璃体液和血浆样本以验证生物标志物。因此，确定了一些候选生物标志物，用于进一步的应用。
