Licier Rígel, Miranda Eric, Serrano Horacio
Department of Medicine, San Juan Bautista School of Medicine, Caguas 00727, Puerto Rico.
Quantitative Proteomics Laboratory, Comprehensive Cancer Center of Puerto Rico, San Juan 00936, Puerto Rico.
Proteomes. 2016 Oct 17;4(4):31. doi: 10.3390/proteomes4040031.
The proper handling of samples to be analyzed by mass spectrometry (MS) can guarantee excellent results and a greater depth of analysis when working in quantitative proteomics. This is critical when trying to assess non-traditional sources such as ear wax, saliva, vitreous humor, aqueous humor, tears, nipple aspirate fluid, breast milk/colostrum, cervical-vaginal fluid, nasal secretions, bronco-alveolar lavage fluid, and stools. We intend to provide the investigator with relevant aspects of quantitative proteomics and to recognize the most recent clinical research work conducted with atypical samples and analyzed by quantitative proteomics. Having as reference the most recent and different approaches used with non-traditional sources allows us to compare new strategies in the development of novel experimental models. On the other hand, these references help us to contribute significantly to the understanding of the proportions of proteins in different proteomes of clinical interest and may lead to potential advances in the emerging field of precision medicine.
在定量蛋白质组学研究中，正确处理用于质谱分析（MS）的样本，能够确保获得出色的结果，并实现更深入的分析。当试图评估耳垢、唾液、玻璃体液、房水、眼泪、乳头抽吸液、母乳/初乳、宫颈-阴道液、鼻分泌物、支气管肺泡灌洗液和粪便等非传统样本来源时，这一点至关重要。我们旨在为研究人员提供定量蛋白质组学的相关知识，并了解利用非典型样本进行的最新临床研究工作，以及通过定量蛋白质组学进行分析的情况。以针对非传统样本来源所采用的最新且不同的方法为参考，有助于我们在新型实验模型开发中比较新策略。另一方面，这些参考资料有助于我们极大地增进对临床相关不同蛋白质组中蛋白质比例的理解，并可能推动精准医学这一新兴领域取得潜在进展。
