Medical College of Nankai University, Tianjin, China, 300071.
Toxicol Mech Methods. 2007;17(8):483-8. doi: 10.1080/15376510601166384.
ABSTRACT Previous studies have reported that chlorotoluron has adverse effects on male mice. Few studies, however, have investigated the mechanism of the action of these herbicides. The study of the effects of chlorotoluron and atrazine ingestion on male mice reproductive function during a critical developmental period, the peripubertal period, is reported here. Chlorotoluron and/or atrazine were administered by PO gavage to mice from day 27 to day 52. The decreased weight ratio of mouse testis to body weight resulted in a reduction in the relative testis weight. Histological study of testis showed that the seminiferous epithelium arrayed loosely and disorderedly, spermatogenic cells became shed, and fewer layers were formed. With the use of an electron microscope, it was noted that mitochondria in the seminiferous epithelium appeared vacuolated; karyotheca swelled and bent. In addition, the number of Sertoli cells was reduced, and part of the tight junction was destroyed. The results of the present study demonstrated that herbicides in all administrated doses caused detrimental changes in mice. The toxic effects of chlorotoluron were more potent than those of atrazine when administrated alone. Moreover, the two compounds have synergistic toxic effects with combination use. All dose paradigms induced degeneration and severe pathological changes in a manner of dose response.
摘要 先前的研究报告表明，绿麦隆对雄性小鼠有不良影响。然而，很少有研究探讨这些除草剂的作用机制。本研究报告了在雄性小鼠生殖功能关键发育期——青春期，摄入绿麦隆和莠去津对其的影响。从第 27 天到第 52 天，通过灌胃方式给小鼠施用绿麦隆和/或莠去津。睾丸与体重的重量比降低导致相对睾丸重量减轻。睾丸组织学研究表明，生精上皮排列松散无序，精子细胞脱落，形成的层较少。使用电子显微镜观察到，生精上皮中的线粒体出现空泡化；核仁肿胀弯曲。此外，支持细胞数量减少，部分紧密连接被破坏。本研究结果表明，所有给药剂量的除草剂都对小鼠造成了损害性变化。单独给药时，绿麦隆的毒性作用强于莠去津。此外，这两种化合物联合使用具有协同毒性作用。所有剂量方案都以剂量反应的方式诱导退化和严重的病理变化。
