Department of Marine Biology, Ocean University of China, Qingdao, 266003PR China.
Toxicol Mech Methods. 2005;15(5):351-4. doi: 10.1080/15376520500194684.
The acute cytotoxicity of abamectin to the sperm of rosy barb (Puntius conchonius) was tested. It was found that 79% sperm remained motile at 30 min after activation by hypo-osmotic sodium buffer (HSB), while the motility of sperm exposed to 0.14, 0.28, and 0.42 mu M abamectin for 30 min was reduced to 60%, 35%, and 15%, respectively. Exposure to abamectin at 0.56 mu M decreased the motility of sperm to an even lower value of 5.67% after 20 min of exposure. This indicates that the toxicity of abamectin to rosy barb sperm is both dose dependent and exposure time dependent. The ultrastructural examination showed that most of the sperm exposed to 0.14 mu M abamectin for 30 min remained morphologically normal, while the midpiece and its surrounding mitochondria of the sperm treated with 0.28 mu M abamectin for 30 min were severely damaged. For the sperm treated with 0.42 mu M abamectin for 30 min, the majority of the sperm were abnormal. Sperm heads were dissolved and the midpiece broken. The mitochondria are located in the midpiece of the sperm and may be the target of abamectin toxicity in rosy barb sperm. The results also suggest that rosy barb sperm are a suitable candidate for rapid screening of the acute toxicities of bioorganic pesticides.
阿维菌素对玫瑰无须魮（Puntius conchonius）精子的急性细胞毒性进行了测试。结果发现，经低渗缓冲液（HSB）激活 30 分钟后，79%的精子仍保持活力，而暴露于 0.14、0.28 和 0.42 μM 阿维菌素 30 分钟后，精子的活力分别降低至 60%、35%和 15%。暴露于 0.56 μM 阿维菌素 20 分钟后，精子的活力甚至降低至 5.67%。这表明阿维菌素对玫瑰无须魮精子的毒性具有剂量和暴露时间依赖性。超微结构检查显示，大多数暴露于 0.14 μM 阿维菌素 30 分钟的精子仍保持形态正常，而暴露于 0.28 μM 阿维菌素 30 分钟的精子中段及其周围的线粒体严重受损。暴露于 0.42 μM 阿维菌素 30 分钟的精子中，大多数精子异常。精子头部溶解，中段断裂。线粒体位于精子中段，可能是阿维菌素对玫瑰无须魮精子毒性的靶标。结果还表明，玫瑰无须魮精子是快速筛选生物有机农药急性毒性的合适候选物。
