Department of Marine Biology, Ocean University of China, QingdaoPeople's Republic of China.
Toxicol Mech Methods. 2005;15(3):205-9. doi: 10.1080/15376520590945621.
The FG cell line derived from the gills of flounder paralichthys olivaceus was used to determine the acute cytotoxic effects of the organotin compound bis-(tri-n-butyltin)oxide (TBTO). Its cytotoxic effects were initially measured by three endpoint systems: neutral red (NR) uptake assay, tetrazolium (MTT) assay, and cell protein assay. Results indicated that the doses of TBTO ranging from 1.7 x 10(- 10) to 1.3 x 10(- 7) M were all toxic, and no difference in cytotoxicity was found between the three test systems. The transmission electron microscopic examination of TBTO-exposed cells revealed that their ultrastructures were markedly altered, as evidenced by dilation of mitochondria, breakdown of rough endoplasmic reticulum, nuclear membrane dissolution, and increased level of lysosomes. It is clear that the cells are highly susceptible to TBTO. This renders FG cells one of several choices for rapidly evaluating the acute toxicities of organotin compounds like TBTO. The mode of action of TBTO leading to the cytotoxicity, including the ultrastructural alteration in FG cells, is also proposed.
从牙鲆鳃中分离得到的 FG 细胞系用于测定有机锡化合物双（三丁基锡）氧化物（TBTO）的急性细胞毒性作用。其细胞毒性作用最初通过三个终点系统来衡量：中性红（NR）摄取测定、四唑（MTT）测定和细胞蛋白测定。结果表明，TBTO 的剂量范围从 1.7×10（-10）至 1.3×10（-7）M 均具有毒性，并且这三种测试系统之间的细胞毒性没有差异。暴露于 TBTO 的细胞的透射电子显微镜检查显示，它们的超微结构明显改变，证据为线粒体扩张、粗面内质网破裂、核膜溶解以及溶酶体水平增加。显然，细胞对 TBTO 高度敏感。这使得 FG 细胞成为快速评估 TBTO 等有机锡化合物急性毒性的选择之一。还提出了导致细胞毒性的 TBTO 的作用模式，包括 FG 细胞中的超微结构改变。
