Li H, Zhang S
Department of Marine Biology, Ocean University of Qingdao, People's Republic of China.
Cell Biol Toxicol. 2002;18(4):235-41. doi: 10.1023/a:1016050911012.
FG-9307, a cell line derived from the gill of flounder Paralichthys olivaceus, was used to determine the acute cytotoxic effects of the organophosphorus insecticide methylparathion. The cytotoxic effects of methylparathion were initially measured by three endpoint systems: neutral red (NR) uptake assay, tetrazolium (MTT) assay, and cell protein assay. Results indicated that concentrations of methylparathion ranging from 5 microg/ml to 60 microg/ml were toxic, and there was no significant difference in cytotoxic effects between the three test systems. Thus, the FG-9307 cell line is one of several choices for evaluating the acute toxicities of organophosphorus insecticides such as methylparathion. The ultrastructure of the cells was also studied. It was found that the ultrastructure of the cells was markedly altered by methylparathion, as evidenced by dilation of mitochondria, breakdown of rough endoplasmic reticulum, nuclear necrosis, and production of numerous lysosomes and lipid vacuoles. This appears to be the first report that a marine fish cell line can be used for acute in vitro cytotoxicity evaluation of methylparathion.
FG-9307是一种从牙鲆（Paralichthys olivaceus）鳃部获得的细胞系，用于测定有机磷杀虫剂甲基对硫磷的急性细胞毒性作用。甲基对硫磷的细胞毒性作用最初通过三个终点系统进行测定：中性红（NR）摄取试验、四氮唑（MTT）试验和细胞蛋白试验。结果表明，甲基对硫磷浓度在5微克/毫升至60微克/毫升范围内具有毒性，并且这三个测试系统之间的细胞毒性作用没有显著差异。因此，FG-9307细胞系是评估甲基对硫磷等有机磷杀虫剂急性毒性的几种选择之一。还对细胞的超微结构进行了研究。发现甲基对硫磷使细胞的超微结构发生了显著改变，表现为线粒体扩张、粗面内质网破裂、核坏死以及大量溶酶体和脂质空泡的产生。这似乎是首次报道海洋鱼类细胞系可用于甲基对硫磷的急性体外细胞毒性评估。
