College of Ocean, Hebei Agricultural University, No. 52 Hebei Street, Qinhuangdao, 066003, People's Republic of China.
Environ Monit Assess. 2011 Apr;175(1-4):315-9. doi: 10.1007/s10661-010-1533-1. Epub 2010 Jun 10.
A cell line (FG cells) derived from a gill of the flounder, Paralichthys olivaceus were used to determine the cytotoxic effects of nonylphenol (NP). Cytotoxicity was measured by three endpoint systems: neutral red (NR) uptake assay, methyl thiazolyl tetrazolium (MTT) assay and cell protein assay. The result showed that NP was cytotoxic to FG cells at all tested concentrations, and toxicity increased as the concentration of NP was progressively increased. The 24 h-IC50 values of NP were 39.81, 37.76 and 38.22 μmol/L for NR uptake, MTT assay and cell protein assay, respectively. Moreover, the morphological changes of FG cells were also studied at the concentration of 30 μmol/L for 24 h. Cells morphology were markedly altered by NP observed under a scanning electron microscopy, as evidenced by swelling cells, two and more nucleolus and an increased number of lipid particles. This would suggest that the FG cell line is a suitable bioindicator for the screening of the acute toxicity of NP.
一种从牙鲆鱼鳃中提取的细胞系（FG 细胞）被用于确定壬基酚（NP）的细胞毒性作用。通过三种终点系统来测量细胞毒性：中性红（NR）摄取测定法、甲基噻唑基四唑（MTT）测定法和细胞蛋白测定法。结果表明，NP 在所有测试浓度下对 FG 细胞均具有细胞毒性，且毒性随 NP 浓度的逐渐增加而增加。NP 对 NR 摄取、MTT 测定法和细胞蛋白测定法的 24 h-IC50 值分别为 39.81、37.76 和 38.22 μmol/L。此外，在 30 μmol/L 浓度下还研究了 24 h 时 FG 细胞的形态变化。扫描电子显微镜下观察到，NP 使 FG 细胞的形态发生明显改变，表现为细胞肿胀、两个或更多核仁以及脂滴数量增加。这表明 FG 细胞系是筛选 NP 急性毒性的合适生物指示剂。
