Department of Toxicology, Murray State University, Breathitt Veterinary Center, Hopkinsville, KY.
Toxicol Mech Methods. 2005;15(4):317-21. doi: 10.1080/15376520590968860.
This study was undertaken to determine selamectin residue in dog's blood and in gloves worn while petting dogs after Revolution application. Revolution contains the active ingredient selamectin (a semisynthetic avermectin), which controls endoparasites and ectoparasites, including adult fleas, flea eggs, ticks, heartworms, ear mites, and sarcoptic mange in dogs, for 30 days. Revolution was applied topically on a group of six adult house hold dogs (240 mg selamectin/dog). The gloves worn for 5 min while petting the dogs were collected in glass jars and the blood samples (5 mL/dog) were collected in EDTA tubes at 0 h, 24 h, and 72 h, and at 1, 2, 3, 4, and 5 weeks post-Revolution application for selamectin residue determination. At no time during the study did the dogs show any signs of toxicity, weight loss, or change in body temperature. Extracts of the blood and the gloves were analyzed for selamectin residue using RP-HPLC coupled with a UV detector (246 nm). Selamectin standard used for peak identification and quantitation was purified from Revolution. Selamectin residue was detected in the blood (10.26 +/- 1.06 ng/mL) only at 72 h post-Revolution application, probably due to its poor dermal absorption and rapid elimination from the circulation. In the glove extracts, the highest concentration of selamectin (518.90 +/- 66.80 ppm) was detected 24 h after Revolution application. Transferable residue of selamectin in gloves from dog's coat was detected at a lesser magnitude after 1 week of Revolution application, and that was followed by a further descending trend during the second, third, and fourth weeks. No selamectin residue was detected in the glove extracts after the fifth week. In spite of selamectin's binding to the sebaceous glands of the skin, gloves contained significant transferable residue. Thus, these findings suggest that repeated exposure to selamectin can pose potential health risks, especially to veterinarians, veterinary technologists, dog trainers/handlers, and pet owners.
本研究旨在确定在使用 Revolution 后抚摸狗时狗血液和手套中的塞拉菌素残留。 Revolution 含有活性成分塞拉菌素（一种半合成阿维菌素），可控制内寄生虫和外寄生虫，包括成年跳蚤、跳蚤卵、蜱、心丝虫、耳螨和狗的疥螨，有效期为 30 天。在一组六只成年家养狗（每只狗 240 毫克塞拉菌素）上进行了局部应用 Revolution。在抚摸狗的 5 分钟内收集戴的手套，并将血液样本（每只狗 5 毫升）收集在 EDTA 管中，在 Revolution 应用后 0 小时、24 小时、72 小时以及 1 周、2 周、3 周、4 周和 5 周时进行塞拉菌素残留检测。在研究期间，狗没有表现出任何毒性、体重减轻或体温变化的迹象。使用反相高效液相色谱法（RP-HPLC）结合紫外检测器（246nm）分析血液和手套中的塞拉菌素残留。用于峰识别和定量的塞拉菌素标准品是从 Revolution 中纯化的。 Revolution 应用后 72 小时仅在血液中检测到塞拉菌素残留（10.26 ± 1.06ng/mL），这可能是由于其皮肤吸收不良和从循环中快速消除。在手套提取物中，在 Revolution 应用后 24 小时检测到塞拉菌素的最高浓度（518.90 ± 66.80ppm）。 Revolution 应用 1 周后，从狗的皮毛转移到手套上的塞拉菌素残留量较小，随后在第二、第三和第四周呈下降趋势。在第五周后，在手套提取物中未检测到塞拉菌素残留。尽管塞拉菌素与皮肤的皮脂腺结合，但手套中仍含有大量可转移的残留。因此，这些发现表明，反复接触塞拉菌素可能会带来潜在的健康风险，尤其是对兽医、兽医技术员、训犬师/ handlers 和宠物主人。
