School of Agriculture, Murray State University, 212S Oakley Applied Science Building, Murray, KY.
Toxicol Mech Methods. 2005;15(4):287-91. doi: 10.1080/15376520590968842.
The objective of this investigation was twofold: (1) to determine the transferable residue of imidacloprid in gloves worn while petting experimental household dogs after the application of Advantage(R) and (2) to determine the imidacloprid residue in the dog's blood. Advantage(R) contains 9.1% imidacloprid, which controls fleas on dogs for up to 30 days. Imidacloprid produces toxicity by interacting with nicotinic receptors. Advantage(R) (364 mg imidacloprid/dog) was applied topically to six household dogs. The glove and blood samples were collected at 24 h, 72 h, and then on a weekly basis for 5 weeks post-Advantage(R) application. The glove samples were collected by petting each dog for 5 minutes while wearing a different glove per dog. The blood samples (5 mL from each dog) were collected into EDTA tubes. The imidacloprid residue was determined in the blood extracts and glove samples using RP-HPLC. The highest levels of imidacloprid residues were detected at the 24-h interval in both glove (254.16 +/- 25.49 ppm) and blood (54.06 +/- 3.00 ppb) samples. The blood imidacloprid residue was reduced by one third at the 72-h interval (18.73 +/- 2.00 ppb) and was not detected after 1 week. Imidacloprid residue in the glove samples decreased approximately one third between each collection interval. The residue of imidacloprid in the glove extract by the fourth week was very low (0.08 +/- 0.02 ppm) and not detected by the fifth week. The present findings suggest that following topical application of Advantage(R), imidacloprid residue can be detected in the dog's blood for up to 72 h, and transferable residue on the dog's coat can be detected for up to 4 weeks. Repeated chronic exposure to imidacloprid may pose possible health risks to veterinarians, veterinary technologists, dog caretakers, and owners.
（1）确定在给实验性家庭犬使用 Advantage(R) 后抚摸犬时所戴手套上残留的可转移的吡虫啉；（2）确定犬血液中的吡虫啉残留量。Advantage(R) 含有 9.1%的吡虫啉，可控制犬身上的跳蚤长达 30 天。吡虫啉通过与烟碱型乙酰胆碱受体相互作用产生毒性。给六只家庭犬应用 Advantage(R)（每只犬 364 毫克吡虫啉）。在应用 Advantage(R)后 24 小时、72 小时和接下来的每周收集一次，共 5 周，收集手套和血液样本。通过抚摸每只犬 5 分钟，同时为每只犬佩戴不同的手套来收集手套样本。从每只犬采集 5 毫升血液样本到 EDTA 管中。使用反相高效液相色谱法（RP-HPLC）测定血液提取物和手套样本中的吡虫啉残留量。在 24 小时间隔内，手套（254.16 ± 25.49 ppm）和血液（54.06 ± 3.00 ppb）样本中检测到的吡虫啉残留量最高。在 72 小时间隔时，血液中吡虫啉残留量减少了三分之一（18.73 ± 2.00 ppb），1 周后未检测到。在每次采集间隔之间，手套样本中的吡虫啉残留量减少了约三分之一。第四周时，手套提取液中的吡虫啉残留量非常低（0.08 ± 0.02 ppm），第五周未检测到。本研究结果表明，在给犬应用 Advantage(R) 后，可在犬血液中检测到吡虫啉残留长达 72 小时，犬毛上可检测到可转移的残留长达 4 周。兽医、兽医技师、犬护理人员和犬主人反复接触吡虫啉可能会带来潜在的健康风险。
