Department of Ophthalmology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Curr Eye Res. 2010 Jan;35(1):56-62. doi: 10.3109/02713680903374216.
To create a retinal neovascularization experimental model using intravitreal injection of microspheres loaded with latex-derived angiogenic fraction.
Thirty-two albino New Zealand rabbits, divided in 4 groups of 8 animals, were enrolled in this study. Rabbits in groups I, II, and III received one intravitreal injection of PLGA (L-lactide-co-glycolide) microspheres with 10, 30, and 50 microg of latex-derived angiogenic fraction into their right eyes, respectively, and group IV received 0.1 ml of microspheres without the angiogenic fraction. Weekly follow-up with ophthalmoscopy and fluorescein angiography was performed; the rabbits were sacrificed in the 4th week and their eyes processed for light microscopy.
All eyes from group I demonstrated increased retinal vascular tortuosity, observed from 14 days after injection and maintained for 28 days, otherwise without new vessels detection. All group II eyes showed vascular changes similar to group I. Fifty percent of the eyes from group II rabbits developed retinal neovascularization 21 days after injection. All eyes from group III demonstrated significant vascular tortuosity and retinal new vessels 2 weeks after injection, progressing to fibrovascular proliferation and tractional retinal detachment. No vascular changes or retinal new vessels were observed in group IV eyes. Light microscopy confirmed the existence of new vessels previously seen on fluorescein angiography, in retinal sections adjacent to the optic disc, not observed in sections at the same area in the control group.
Thirty- and 50-microg microspheres containing latex-derived angiogenic fraction injected into the vitreous cavity induced retinal neovascularization in rabbits.
通过玻璃体腔注射载有乳胶衍生血管生成部分的微球，建立视网膜新生血管实验模型。
本研究纳入 32 只白化新西兰兔，分为 4 组，每组 8 只。第 I、II、III 组兔右眼分别玻璃体腔注射载有 10、30 和 50μg 乳胶衍生血管生成部分的 PLGA（L-丙交酯-共-乙交酯）微球，第 IV 组注射 0.1ml 不含血管生成部分的微球。每周进行眼科检查和荧光素血管造影随访；第 4 周处死兔子，取眼球行光镜检查。
第 I 组所有兔子的视网膜血管均出现弯曲度增加，在注射后 14 天即可观察到，并持续至 28 天，无新生血管出现。第 II 组所有兔子的眼部血管均发生与第 I 组类似的变化。第 II 组 50%的兔子在注射后 21 天出现视网膜新生血管。第 III 组所有兔子的眼部均在注射后 2 周出现明显的血管弯曲和视网膜新生血管，进展为纤维血管增殖和牵引性视网膜脱离。第 IV 组兔子的眼部未见血管变化或视网膜新生血管。光镜检查证实了在注射后荧光素血管造影中观察到的新血管的存在，在靠近视盘的视网膜切片中观察到，在对照组相同区域的切片中未观察到。
玻璃体腔注射 30 和 50μg 载有乳胶衍生血管生成部分的微球可诱导兔视网膜新生血管形成。
