兔视网膜下新生血管形成的一种新模型。
A new model of subretinal neovascularization in the rabbit.
作者信息
Kimura H, Sakamoto T, Hinton D R, Spee C, Ogura Y, Tabata Y, Ikada Y, Ryan S J
机构信息
Doheny Eye Institute, Los Angeles, CA 90033, USA.
出版信息
Invest Ophthalmol Vis Sci. 1995 Sep;36(10):2110-9.
PURPOSE
To establish a new model of subretinal neovascularization (SRN) in the rabbit by implanting basic fibroblast growth factor (bFGF)-impregnated gelatin microspheres beneath the retina.
METHODS
Basic fibroblast growth factor-impregnated gelatin microspheres were prepared by forming a polyion complex between gelatin and bFGF. The microspheres, containing 2.5 micrograms of bFGF, were injected into the subretinal space of rabbit eyes (n = 29). Control eyes (n = 10) received bFGF-free gelatin microspheres. Eyes were followed up for 3 days to 8 weeks by ophthalmoscopy, photography, fluorescein angiography, light microscopy, and transmission electron microscopy.
RESULTS
Twenty of 24 experimental eyes (83%) showed fluorescein leakage from SRN 2 weeks after implantation of the bFGF-impregnated microspheres. This leakage continued for 2 to 6 more weeks. In striking contrast, control eyes showed no fluorescein leakage. Histologic examination revealed SRN in all the experimental eyes but in none of the control eyes.
CONCLUSIONS
Subretinal implantation of bFGF-impregnated gelatin microspheres induces reproducible SRN in the rabbit.
目的
通过在兔视网膜下植入含碱性成纤维细胞生长因子（bFGF）的明胶微球，建立一种新的兔视网膜下新生血管形成（SRN）模型。
方法
通过明胶与bFGF形成聚离子复合物制备含碱性成纤维细胞生长因子的明胶微球。将含有2.5微克bFGF的微球注入兔眼（n = 29）的视网膜下间隙。对照眼（n = 10）注入不含bFGF的明胶微球。通过检眼镜检查、摄影、荧光素血管造影、光学显微镜和透射电子显微镜对眼睛进行3天至8周的随访。
结果
24只实验眼中的20只（83%）在植入含bFGF的微球后2周出现视网膜下新生血管的荧光素渗漏。这种渗漏持续2至6周以上。与之形成鲜明对比的是，对照眼未出现荧光素渗漏。组织学检查显示所有实验眼中均有视网膜下新生血管形成，而对照眼中均未出现。
结论
视网膜下植入含bFGF的明胶微球可在兔眼中诱导出可重复的视网膜下新生血管形成。
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